Triptolide-Mediated Apoptosis by Suppression of Focal Adhesion Kinase through Extrinsic and Intrinsic Pathways in Human Melanoma Cells
Figure 5
Effect of TPL on the level of FAK and FAK interaction with RIP in SK-MEL-2 cells. (a) Cells were treated with 0, 30, 50, and 70 nM of TPL, and then cells were collected. Cell lysates were analyzed by Western blotting. Each protein was detected using p-FAK, FAK antibodies. The relative abundance of each p-FAK band to its own FAK plus cleaved FAK was quantified by densitometry, and the control levels were set to 1. (b) Cells were either not treated or treated with TPL (50 nM) for 0, 12, and 24 h, and lysates were immunoprecipitated and immunoblotted as indicated.
