Inhibitory Effects of Baicalin on the Expression and Activity of CYP3A Induce the Pharmacokinetic Changes of Midazolam in Rats
Figure 7
Reversible inhibition of CYP3A by baicalin in rat liver microsomes. (a) Lineweaver-Burk plot of the inhibitory effect of baicalin on CYP3A-mediated MDZ hydroxylation activity. The reciprocal of MDZ disappearance is plotted versus the reciprocal of MDZ concentration in the presence and absence of the inhibitor baicalin (0, 20, 40, 80, 160, and 320 μM). The “” represents the velocity of the disappearance of MDZ. MDZ was used at concentrations of 12.5, 25, and 50 μM. (b) Secondary plot of the slopes from the Lineweaver-Burk plot versus baicalin concentrations. BA: baicalin.
