Anti-Diabetic Activities of Jiaotaiwan in db/db Mice by Augmentation of AMPK Protein Activity and Upregulation of GLUT4 Expression

Figure 1

HPLC profiles of JTW and reference material of berberine hydrochloride. The chromatographic conditions were indicated as follows: chromatographic column, C18 (4.6 × 250 mm², 5 μm); flow rate, 1 mL/min; mobile phase, 0.05 M methyl cyanide and potassium dihydrogen phosphate (25 : 75); column temperature, 30°C; examination wave length, 346 nm; and sample volume, 20 μL. (a) The peak area of the reference material of berberine hydrochloride was 1481.31 and the retention time was 19.39 min. (b) The peak area of JTW was 1402.66 and the retention time was 19.41 min. Given the formula (𝐴₁/𝐶₁ = 𝐴₂/𝐶₂), the berberine concentration in the three JTW samples was 25.61 mg/mL (𝐴₁=1481.31, 𝐶₁=0.02, and 𝐴₂=1402.66).

(a)

(b)