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Evidence-Based Complementary and Alternative Medicine
Volume 2013 (2013), Article ID 186573, 8 pages
http://dx.doi.org/10.1155/2013/186573
Research Article

Combining Oxymatrine or Matrine with Lamivudine Increased Its Antireplication Effect against the Hepatitis B Virus In Vitro

1China Military Institute of Chinese Medicine, 302 Military Hospital, Beijing 100039, China
2College of Traditional Chinese Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichnan 610075, China
3Pediatric Liver Disease Therapy and Research Center, 302 Military Hospital, Beijing 100039, China
4Integrative Medical Center, 302 Military Hospital, No. 100 4th Ring Road, Beijing 100039, China
5College of Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu 211198, China
6College of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China

Received 1 September 2012; Revised 5 December 2012; Accepted 19 December 2012

Academic Editor: Jae Youl Cho

Copyright © 2013 Zhi-Jie Ma et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Some recent clinical reports have shown that the combination of oxymatrine, a phyto-derived drug, with lamivudine (3TC) could improve its curative effect against hepatitis B virus (HBV) infection. However, the experimental data in support of this combination strategy are lacking. In this study, we investigated the anti-HBV activity of the combination of 3TC and either oxymatrine or matrine on HepG2 2.2.15 in vitro. The activities of the combination and the solo compound, each in different concentrations, were compared on the 3rd, 6th, and 9th experimental days. The cytotoxicity results showed that the nontoxic concentrations of both oxymatrine and matrine to HepG2 2.2.15 cells were 800 μg/mL. We found that the single use of oxymatrine below 100 μg/ml, matrine below 200 μg/ml, and 3TC below 30 μg/ml showed weak inhibitory effects on the secretion of hepatitis B surface antigen (HBsAg), hepatitis B e antigen (HBeAg), and HBV-DNA in culture media; the combination of 3TC (30 μg/ml) with oxymatrine (100 μg/ml) or matrine (100 μg/ml) showed significant inhibitory effects that were higher than or equivalent to the single use of 3TC at 100 μg/ml. The results provide a new impetus to develop novel, multicomponent anti-HBV drugs through the combination of natural products with nucleoside analogs to enhance their activity.