The Action of Antidiabetic Plants of the Canadian James Bay Cree Traditional Pharmacopeia on Key Enzymes of Hepatic Glucose Homeostasis
Figure 3
Effect of 17 plant extracts on kinases regulating G6Pase activity. (a) Phosphorylation of Akt. Phosphorylated (p-Akt) and total Akt were measured by Western blot in H4IIE cells treated with optimal nontoxic concentrations of indicated plant extracts. The ratio of p-Akt/total Akt is expressed as a percentage relative to values obtained for DMSO (0.1%) vehicle controls. Insulin (100 nM) was used as a positive control. significantly different from DMSO vehicle control. (b) Phosphorylation of AMPK. Phosphorylated (p-AMPK) and total AMPK were measured by Western blot in H4IIE cells treated with optimal nontoxic concentrations of indicated plant extracts. The ratio of p-AMPK/total AMPK is expressed as a percentage relative to values obtained for DMSO (0.1%) vehicle controls. AICAR (2 mM) was used as a positive control. significantly different from DMSO vehicle control.
