The Action of Antidiabetic Plants of the Canadian James Bay Cree Traditional Pharmacopeia on Key Enzymes of Hepatic Glucose Homeostasis
Figure 4
Effect of 17 plant extracts on components involved in hepatic glucose storage. (a) Activation of GS. Results shown represent the glycogen synthase (GS) activity observed after overnight treatment of HepG2 cells with optimal nontoxic concentrations of indicated plant extracts. They are expressed relative to DMSO (0.1%) vehicle controls (100% activity). Assays were carried out in triplicate on three different cell cultures. Insulin (100 nM) and AICAR (2 mM) were used as positive controls. significantly different from DMSO vehicle control. (b) Phosphorylation of GSK-3. Phosphorylated (p-GSK-3) and total GSK-3 were measured by Western blot in HepG2 cells treated with optimal nontoxic concentrations of indicated plant extracts. The ratio of p-GSK-3/total GSK-3 is expressed as a percentage relative to values obtained for DMSO (0.1%) vehicle controls. Insulin (100 nM) and AICAR (2 mM) were used as positive controls. and significantly different from DMSO vehicle control. Inset: Correlation between GSK-3 phosphorylation and activation of GS induced by the 17 plant extracts (, ).
