Suppressions of Migration and Invasion by Cantharidin in TSGH-8301 Human Bladder Carcinoma Cells through the Inhibitions of Matrix Metalloproteinase-2/-9 Signaling
Figure 3
Wound healing assay for the effects of cantharidin on the migration of TSGH-8301 cells. Cells were placed for 24 h; then a wound was performed by scraping confluent cell layers with a pipette tip. Cantharidin was added to the cells at the final concentration were 0, 1 and 2.5 μM and then incubated for 24. (a) Some of the representative photographs of invading treated and untreated cells are presented. Three separate experiments with similar results were carried out. (b) The migrated cells in the five random fields after exposure for 24 h were counted to quantify, and data was expressed as mean ± S.D. on the basis of untreated cells (control) represented as 100%. , significant difference between cantharidin-treated groups and the control as analyzed by Dunnett’s posttest.
