Suppressions of Migration and Invasion by Cantharidin in TSGH-8301 Human Bladder Carcinoma Cells through the Inhibitions of Matrix Metalloproteinase-2/-9 Signaling
Figure 5
Cantharidin affects the levels of associated proteins and gene levels in migration and invasion of TSGH-8301 cells. Cells were treated with cantharidin (0, 1, and 2.5 μM) for 24 h, and then cells were collected. The total protein extract was quantified and determined as described in Section 2. The levels of p-p38, p-JNK1/2, p-ERK1/2, MMP-2, and MMP-9 protein expressions (a) were estimated by Western blotting as described in Section 2. (b) The total RNA was extracted from cantharidin-treated cells, and the RNA samples were reverse-transcribed to cDNA for real-time PCR as described in Section 2. The ratios between MMP-2, MMP-9, and GAPDH mRNA are used and data represents mean ± SD in duplicate of at least three independent experiments. was considered significantly as analyzed by Dunnett’s posttest.
