Research Article

Serum Containing Tao-Hong-Si-Wu Decoction Induces Human Endothelial Cell VEGF Production via PI3K/Akt-eNOS Signaling

Figure 7

Effects of 10% TSD medicated serum on expression of p-Akt, total Akt, and p-eNOS in presence of LY294002 and L-NAME. Cells were treated with 10% blank serum or 10% TSD medicated serum for 24 h in presence of LY294002, L-NAME, or both. Cells were lysed, and the expression was determined by western blot with specific primary antibody to p-Akt, total Akt, and p-eNOS; bands were displayed with secondary antibody and ECL system. Data were expressed as mean ± SD, . and compared with p-eNOS of 10% blank serum group, and compare with p-Akt of 10% blank serum group.
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