Zeaxanthin Induces Apoptosis in Human Uveal Melanoma Cells through Bcl-2 Family Proteins and Intrinsic Apoptosis Pathway

Figure 2

Dose effect of zeaxanthin on cell viability of uveal melanoma cells, uveal melanocytes, retinal pigment epithelial (RPE) cells, and fibroblasts. Human normal uveal melanocytes (a), RPE cells (b), and fibroblasts (c) or uveal melanoma cells C918 (d) and SP6.5 (e) were seeded into 96-well plates and treated with zeaxanthin at various doses for 48 h and cell viability was determined by MTT assay (see Section 2). Zeaxanthin at all tested concentrations selectively reduced the cell viability of uveal melanoma cells, without affecting cell viability of uveal melanocytes, RPE cells, and fibroblasts. Data are mean ± SD (𝑛=3). ^∗𝑃<0.05, versus control (cells cultured without zeaxanthin).

(a)

(b)

(c)

(d)

(e)