Zeaxanthin Induces Apoptosis in Human Uveal Melanoma Cells through Bcl-2 Family Proteins and Intrinsic Apoptosis Pathway
Figure 7
Effects of zeaxanthin on the activation of caspase-3, -8, and -9 in human uveal melanoma cell lines. Uveal melanoma cells (C918) were treated with various concentrations of zeaxanthin (10–100 μM) for 2 h. Cells were collected and lysed (see Section 2). The caspase-3, -8, and -9 in the lysates were measured using relevant caspase colorimetric assay kits. Caspase-3, -8, and -9 levels in zeaxanthin-treated cells at different concentrations were expressed as percentage of the controls. Zeaxanthin significantly increased caspase-3 (a) and caspase-9 (c) activities in a dose-dependent manner, but not caspase-8 activities (b). Data are mean ± SD (). , versus control (cells cultured without zeaxanthin).
