Discovery of Endothelium and Mesenchymal Properties of Primo Vessels in the Mesentery

Figure 2

Immunofluorescent staining of primo vessels in the mesentery with endothelial markers, fibroblast markers, and phalloidin in longitudinal sections. Endothelial and fibroblast markers expressed in primo vessels in the mesentery. Antibodies to endothelial markers (vWF, CD31, VE-cadherin), fibroblast markers (PDI, MST1, procollagen I), and lymphatic endothelial marker (LYVE-1) (green) were used. DAPI (blue) and phalloidin (red) were used to identify primo vessels (yellow dashed area). Both of endothelial markers and fibroblast markers (green) were expressed in primo vessels. Lymphatic endothelial marker was negative in primo vessels.