Antiobesity Effect of Codonopsis lanceolata in High-Calorie/High-Fat-Diet-Induced Obese Rats
Figure 3
The effect of wCL on 3T3-L1 adipocyte differentiation. (a) 3T3-L1 cells were differentiated with MDI in the absence or presence of wCL (0, 0.3, 0.5, 0.7 mg/mL) for eight days, followed by measurement of lipid contents by Oil Red O staining. (b) Stained oil droplets were dissolved with isopropanol and quantified by spectrophotometric analysis at 510 nm. The results were represented as relative lipid contents. *, ** by SPSS compared to MDI-treated cells. (c) Expression of C/EBP and PPAR mRNA from 3T3-L1 cells as described in (a) was measured by RT-PCR.