The Effects of Brazilian Green Propolis against Excessive Light-Induced Cell Damage in Retina and Fibroblast Cells
Figure 3
Effects of propolis on cell damage induced by UVA irradiation in an NB1-RGB culture. (a) Representative fluorescence microscopic images show nuclear staining for Hoechst 33342 and PI after UVA 10 J/cm2 irradiation. Upper photomicrographs show Hoechst 33342 and lower ones propidium iodide (PI) staining at 6 h after UVA irradiation. (b) The number of cells exhibiting PI fluorescence was counted, and positive cells were expressed as the percentage of PI to Hoechst 33342. Pretreatment of cells with propolis (30 μg/mL) significantly reduced the amount of cell death (versus cells treated with UVA irradiation alone). (c) Cell viability was assessed by immersing cells in WST-8 solution for 6 h at 37°C, with absorbance recorded at 492 nm. UVA induced a decrease in cell viability. Propolis concentration-dependently inhibited UVA-induced cell damage. Data are shown as means ± S.E.M. (). *, ** versus UVA exposure plus the vehicle-treated group, and ## versus control. Scale bar represents 100 μm.
