The Effects of Brazilian Green Propolis against Excessive Light-Induced Cell Damage in Retina and Fibroblast Cells

Figure 5

Effects of propolis on intracellular ROS production induced by UVA or white light irradiation in an NB1-RGB or 661W culture. The NB1-RGB or 661W culture was treated with propolis for 1 h, and this was supplemented with CM-H₂DCFDA at 10 μM for 20 min. ROS production was stimulated with UVA 10 J/cm² or 3,000lx of white light, and fluorescence was measured at 0–20 min. UVA or white light irradiation induced oxidation of DCFH in NB1-RGB or 661W culture. Intracellular ROS production was increased by UVA or white light irradiation, while it was concentration-dependently reduced by propolis treatment. Data are shown as means ± S.E.M. (𝑛=6). *𝑃<0.05, **𝑃<0.01 versus UVA or white light exposure plus the vehicle-treated group, and ^##𝑃<0.01 versus control.

(a)

(b)

(c)