The Effects of Brazilian Green Propolis against Excessive Light-Induced Cell Damage in Retina and Fibroblast Cells
Figure 6
Effects of propolis on UVA-induced expression of phosphorylated p38 and ERK1/2 and MAPK inhibitors in an NB1-RGB culture. Representative Western blots (a) showing activation of p38 and ERK in the nontreated, UVA 10 J/cm2 exposure plus vehicle-treated, and UVA exposure plus propolis-treated (30 μg/mL) groups. Phosphorylation of p38 (b) and ERK1/2 (c) was determined by immunoblotting assay. Quantitative analysis of the band density of p38 (b) and ERK (c) by densitometric analysis. These intensities were normalized with total p38 and total ERK, respectively. (d) Cell viability was assessed by immersing cells in WST-8 solution for 6 h at 37°C, with absorbance recorded at 492 nm. SB203580 and U0126 inhibited the decrease of cellular viability induced by UVA, and the level was as high as of a propolis-treated group. Data are shown as means ± S.E.M. (). ** versus UVA exposure plus vehicle-treated cells and ## versus control.
