Pomegranate Juice Metabolites, Ellagic Acid and Urolithin A, Synergistically Inhibit Androgen-Independent Prostate Cancer Cell Growth via Distinct Effects on Cell Cycle Control and Apoptosis
Figure 5
Effect of UA and EA on apoptosis. PC-3 and DU-145 cells were cultured to 85% of confluence in 100 mm dishes and treated with increasing concentrations of EA or with UA for 72 hours. Adherent and floating cells were collected. Cells were double stained with Annexin V/PI and subjected to flow cytometry assay. Representative Annexin V/PI flow cytometry of PC-3 and DU-145 treated with DMSO control, EA 45 μmol/L, and UA 90 μmol/L (a). Percentage of apoptotic cells of PC-3 and DU-145 treated for 72 hours with 15, 30, and 45 μmol/L of EA or with 30, 60, and 90 μmol/L of UA (b, c). The data are mean ± SD of three independent experiments; *significantly different from DMSO control, .
