Research Article

In Vitro Morphological Assessment of Apoptosis Induced by Antiproliferative Constituents from the Rhizomes of Curcuma zedoaria

Figure 4

Representative composite images show morphological changes of selected cancer cells (MCF-7, Ca Ski, and HCT-116) detected with dual staining of Hoechst 33342/PI. Cells were treated with curzerenone for 48 h and imaged by fluorescence microscope (magnification 630x). Arrows indicate (1) viable cells with normal nuclei, (2) live cells with apoptotic nuclei, (3) dead cells with normal nuclei, and (4) dead cells with apoptotic nuclei.
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