BlueBerry Isolate, Pterostilbene, Functions as a Potential Anticancer Stem Cell Agent in Suppressing Irradiation-Mediated Enrichment of Hepatoma Stem Cells
Figure 3
Pterostilbene treatment decreased the percentage of CD133+ Mahlavu cells. (a) Irradiated Mahlavu cells were treated with different concentrations of pterostilbene and analyzed for the abundance of CD133+ cells. Dose-dependently, pterostilbene decreased the CD133+ subpopulation cells. (b) Pterostilbene disrupted the tumor sphere-forming ability of CD133+ Mahlavu cells. When pterostilbene was added into the culture medium of CD133+ Mahlavu cells, pterostilbene dose-dependently prevented Mahlavu tumor sphere formation. (c) Total protein lysates were obtained from pterostilbene-treated Mahlavu tumor spheres for western blot analysis. The expression levels of stemness markers such as CD133, c-Myc, and proinflammation marker COX-2 were suppressed by the addition of pterostilbene in a dose-dependent manner, while proapoptosis marker PARP was increased under pterostilbene treatment.
