β-Elemene-Attenuated Tumor Angiogenesis by Targeting Notch-1 in Gastric Cancer Stem-Like Cells
Figure 2
(a) FACS sorting results of cultured MKN-45 cells. (b) MTT assay revealed an enhanced proliferation capacity of CD44+ cells compared to CD44− cells 48 and 72 hours after sorting ( and , resp.). (c) Western blot of the cells 48 h after FACS sorting, which indicates a remarkable difference in Notch-1 and Hes1 expressions between CD44+ and CD44− cells. (d) Spheroid colony formation by CD44+ and CD44− cells. (e) Quantitive analysis of the spheroid clone formation efficacy of CD44+ and CD44− cells.
