Bioactive Lipidic Extracts from Octopus (Paraoctopus limaculatus): Antimutagenicity and Antiproliferative Studies

Table 3

Anti-mutagenic potential of AFB₁^a -spiked fractions obtained from thin layer chromatography fractionation of fraction RB2 (𝑅_𝑓 = 0.78–1.00) from TLC 2 (average^b TA100 and TA98 revertants/plate, with S9).

Extract fraction^c	Fraction dilution^c
	1×10^0	1×10^−1	1×10^−2	1×10^−3
TA98
RB21 (𝑅𝑓 = 0.00–0.47)	49±9	73±4	257±20	325±24
RB22 (𝑅𝑓 = 0.47–0.79)	71±3	141±20	219±19	218±28
RB23 (𝑅𝑓 = 0.79–1.00)	18±2	175±13	236±18	265±21
TA100
RB21 (𝑅𝑓 = 0.00–0.47)	175±30	198±18	220±15	275±71
RB22 (𝑅𝑓 = 0.47–0.79)	292±12	455±31	843±27	883±12
RB23 (𝑅𝑓 = 0.79–1.00)	320±25	695±40	567±44	1687±70

^aAFB₁ (used as positive control) tested at 500 ng/plate induced 1006 ± 83 and 1689 ± 78 revertants/plate for TA98 and TA100, respectively. Spontaneous revertants were 55±3 and 188±14 for TA98 and TA100, respectively.
^bValues are means of three replicates ± SEM.
^cExtracts were diluted and spiked with enough toxin to yield 500 ng of pure AFB₁ plate.