- About this Journal
- Abstracting and Indexing
- Aims and Scope
- Annual Issues
- Article Processing Charges
- Articles in Press
- Author Guidelines
- Bibliographic Information
- Citations to this Journal
- Contact Information
- Editorial Board
- Editorial Workflow
- Free eTOC Alerts
- Publication Ethics
- Reviewers Acknowledgment
- Submit a Manuscript
- Subscription Information
- Table of Contents
Evidence-Based Complementary and Alternative Medicine
Volume 2013 (2013), Article ID 273712, 9 pages
Loranthus micranthus Linn.: Biological Activities and Phytochemistry
1Biomolecular Research Group, Biochemistry Program, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
2Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
3Department of Medical Microbiology, Tropical Infectious Disease Research and Education Center (TIDREC), Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
Received 29 April 2013; Accepted 4 August 2013
Academic Editor: Mohamad Fawzi Mahomoodally
Copyright © 2013 Soheil Zorofchian Moghadamtousi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Loranthus micranthus Linn. is a medicinal plant from the Loranthaceae family commonly known as an eastern Nigeria species of the African mistletoe and is widely used in folkloric medicine to cure various ailments and diseases. It is semiparasitic plant because of growing on various host trees and shrubs and absorbing mineral nutrition and water from respective host. Hence, the phytochemicals and biological activities of L. micranthus demonstrated strong host and harvesting period dependency. The leaves have been proved to possess immunomodulatory, antidiabetic, antimicrobial, antihypertensive, antioxidant, antidiarrhoeal, and hypolipidemic activities. This review summarizes the information and findings concerning the current knowledge on the biological activities, pharmacological properties, toxicity, and chemical constituents of Loranthus micranthus.
Loranthus micranthus (L. micranthus) as member of the Loranthaceae family is the eastern Nigeria species of the African mistletoe. Mistletoes are the semiparasitic plants because they normally grow on various host trees and shrubs and they are dependent on their respective host for mineral nutrition and water, although they produce their own carbohydrates through photosynthesis . Mistletoe was described as “an all purpose herb” due to its rich traditional uses and it has been widely used in ethnomedicine for various purposes, including antihypertensive, anticancer, antispasmodic, and antidiabetic, and for treatment of epilepsy, headache, infertility, menopausal syndrome and rheumatism [2, 3]. Previous studies demonstrated that composition and hence biological activities of mistletoe are dependent on harvesting period and host tree species [4–7]. Nigeria has wide distribution of mistletoes with different local names that depend on the area where they occur. Loranthus micranthus represents Eastern Nigeria mistletoes that mostly grow in the southeastern region of the country. It grows on various host trees including Persia americana, Baphia nitida, Kola acuminata, Pentaclethra macrophylla, and Azadirachta indica [8, 9]. L. micranthus has been widely used in folk medicine as antimicrobial, antihypertensive, anticancer, and antidiabetic agent, for treatment of headache, infertility, epilepsy, cardiovascular diseases, menopausal syndrome, agglutination, and rheumatism, and also in conditions that generally require immunomodulatory. Some of these ethnomedicinal uses have already been supported and acclaimed by several investigations [1, 2, 10, 11]. In Nigeria and South Africa, L. micranthus has been widely used as ethnomedicine for treatment of hypertension, diabetes, and schizophrenia and as an immune system booster .
Extensive phytochemical evaluations of L. micranthus extracts demonstrated the presence of various phytoconstituents and compounds. Crude methanolic extract from leaves of L. micranthus harvested from P. americana was found to possess terpenoids, steroids, oils, proteins, resins, flavonoids, tannins, saponins, alkaloids, reducing sugar, acidic compounds, glycosides, and carbohydrates. Alkaloids showed the highest presence in abundance. The weakly acidic fraction analysis isolated from aqueous methanolic extract of leaves of L. micranthus showed the presence of terpenoids, steroids, acidic compounds, flavonoids, and carbohydrate. Lower rate of flavonoids and carbohydrates elicited in comparison with methanolic extract, while the amount of terpenoids, acidic compounds, and steroids remained unchanged . Chemical composition of L. micranthus was found to be seasonal dependent. During April, the onset of rainy season, methanolic extract of the leaves harvested from P. Americana showed higher concentrations of carbohydrates, acidic compounds, steroids, and alkaloids compared to July, the time for peak of rainy season. Interestingly, Tannins, saponins, glycosides, and reducing sugars were not found in July samples. However, higher amounts of flavonoids and oils were detected in July samples compared to April ones . Iwalokun and colleagues  have investigated the phytochemicals such as n-butanol, chloroform, ethyl acetate, and water fractions of the methanolic extract of L. micranthus leaves of Kola nut tree (K. acuminata). Moderate and high levels of steroids and terpenoids were detected in n-butanol fraction, while phenolics, reducing sugars, and tannins were detected in all fractions together with moderate level for phenolics and tannins in chloroform fraction. Indeed, flavonoids and saponins were only present in ethyl acetate and water fractions, respectively. They have demonstrated that terpenoids were present in low-to-moderate levels in chloroform and water fractions, while they were not detected in ethyl acetate fraction. Phytochemical studies on L. micranthus leaves harvested from six different host trees, namely, P. americana, B. nitida, K. acuminata, P. macrophylla, A. indica, and Irvingia gabonensis, revealed that alkaloids are preponderant in the extracts of K. acuminata, P. Americana, and I. gabonensis. Moreover, the phytochemical constituent host dependency was also shown [31, 32]. A study on petroleum ether extract of L. micranthus leaves parasitic on P. americana harvested at different seasons (January, April, July, and November) showed only presence of alkaloids in April and July and proved harvesting period dependency in phytochemicals of L. micranthus . In several studies attempting to identify the active compounds responsible for various biological activities of L. micranthus, especially immunomodulatory activity, a variety of compounds (Table 1) were isolated and their structures were characterized (Figure 1).
3. Biological Activities
Fractions, pure compounds, and crude extracts of plants are precious and crucial resources for effective molecules in treatment of different diseases and ailments in animal and human . L. micranthus has been found to possess antidiabetic, antimicrobial, antihypertensive, hypolipidemic, antioxidant, antidiarrhoeal, and immunomodulatory activities. A study by Edem and Usoh  demonstrated that the use of L. micranthus is safe and without adverse biochemical effects or hepatocellular damages in rats. They have administered different doses of the water extract of L. micranthus leaves from 275 to 827 mg/kg for 21 days on male albino Wistar rats. Blood samples analysis did not reveal any significant changes in the level of protein, urea, glucose, bilirubin, cholesterol, alkaline phosphatase, and aspartate transaminase. However, significant reduction was observed in the level of alanine transaminase enzyme by using 551 and 827 mg/kg of the extract. However, an in vitro study on aqueous leaf extract of L. micranthus indicated cytotoxic, genotoxic, and mitodepressive effects of that extract against Allium cepa root cells especially at dose beyond pharmacological range . They showed a significant inhibition of root growth with an effective concentration (EC50) of 28.2 mg/mL. The extract revealed dose-dependent decrease in mitotic index by 2.4–27.4% using a range from 5 to 40 mg/mL of the extract except at 2.5 mg/mL in which 11.9% elevation was reported compared to 50% decrease for sodium azide used at 100 μg/mL as positive control. Hence, for safety reasons, using the lower concentrations of L. micranthus for human phytomedicine in prolonged use coupled with in vivo genotoxic tests is suggested .
3.1. Immunomodulatory Activity
Stimulation of human immune system has been identified as a possible pathway to inhibit the progression of some diseases without eliciting adverse side effects [37, 38]. However, immune system stimulation is a desired response if overall process culminates with cure or faster convalescence from sickness [39, 40]. One folkloric use of L. micranthus is due to its ability to enhance the immune system with concomitant quicker convalescence . An in vivo study on aqueous-methanol extract from L. micranthus leaves harvested from five different host trees has investigated the immunomodulatory activity in rat and mice models, including the cyclophosphamide-induced myelosuppression (CIM) test, the humoral mediated antibody titration (AT) test, total and differential leukocyte count (TLC and DLC), and the cellular mediated delayed-type hypersensitivity reaction (DTHR) test . According to the results, the extracts (with overall order of activity from K. acuminate > Citrus spp. > P. Americana > Parkia biglobosa > P. macrophylla) were found to be potent and safe complementary or alternative medicines to cure the immunodeficiency conditions without any toxicity (LD50 values > 5000 mg/kg for acute toxicity tests). Leaves extract parasitic on K. acuminate at a dose of 200 mg/kg caused 139.69% stimulation of total leukocyte in mice compared to 75.35% increase for levamisole (25 mg/kg) as a standard immunostimulatory drug. At dose of 100 mg/kg, it also showed 175% stimulation on DTHR in immunocompetent mice compared to 122.50% increase for levamisole as positive control. 71.35% and 81.53% of primary and secondary stimulation on antibody titration in rats at a dose of 100 mg/kg of leaves extract also exhibited the significant immunomodulatory effect compared to 24.50% and 0.40% of primary and secondary stimulation for levamisole (25 mg/kg), respectively [10, 41]. Results from another study by the same group showed that ethanol and n-hexane leaves extracts parasitic on P. americana at doses from 100 to 400 mg/kg also exhibited a dose-dependent immunomodulatory activity assessed by DTHR and CIM models in mice. Results showed over 170% of stimulation for both extracts at 400 mg/kg dose . The immunomodulatory analysis of five different extracts of L. micranthus leaves, parasitic on K. acuminata, namely, n-hexane, chloroform, ethyl acetate, acetone, and methanol by DTHR test in experimental mice and their phytochemical evaluation of the fractions, demonstrated that the most active fractions were either mainly terpenoidal, flavonoidal, or steroidal . The results also confirmed the most significant immunostimulatory effects for chloroform extract with 311.11% and 122.22% enhancement in stimulation using 250 and 500 mg/kg of the extract, respectively. The compounds 12–14 at dose of 100 μg/mL showed increasing effects on C57BL/6 mice splenocytes proliferation with 91.49%, 95.17%, and 94.23% stimulation values, respectively, compared to 16.09% stimulation for 10 μg/mL of lipopolysaccharide (LPS) as positive control. However, compounds 12, 13 and 14 exhibited moderate stimulatory effect on CD69 molecule expression . The compound 15 with dose of 100 μg/mL exhibited a weak immunostimulatory activity by inducing 24.44% and 86.98% stimulation of mice splenocytes proliferation and early activation of CD69 molecule, respectively. In addition, it also showed a nonsignificant effect on IL-8 receptor expression . The steroids compounds of 18 and 19 at a dose of 100 μg/mL exhibited significant immunostimulatory activity on the C57BL/6 mice splenocytes with 46% and 43% stimulation, respectively, compared to 7.69% increase for the negative control, although CD69 expression assay revealed minimal stimulation. The compounds 16 and 17 with the same concentration showed weaker stimulations of 30% and 29%, respectively, on the C57BL/6 mice splenocytes . Also, a mild immunostimulatory activity was observed for compound 20 when it was tested on C57BL/6 splenocytes . However, 69.84% and 56.34% stimulation elicited from compounds 21 and 22 at a dose of 25 μg/mL for proliferation analysis on mice splenocytes (C57BL/6) compared to 7.58% value for unstimulated control. The CD69 expression assay also exhibited significant proliferation values of 2.71% and 2.31% for compounds of 21 and 22, respectively .
3.2. Antidiabetic Activity
Diabetes mellitus is one of the prevalent and serious chronic diseases around the world . Therefore, finding the promising ways to improve the quality of life for diabetic patients and preventing or reversing diabetic complications necessitate investigations among the arsenal of herbs . Osadebe and colleagues  have studied the anti-diabetic activity of the methanolic extracts of leaves of L. micranthus parasitic on P. americana, B. nitida, K. acuminata, P. macrophylla, and A. indica. The extracts were found to possess significant dose-dependent antihyperglycemic effects in alloxan-induced diabetic albino and normoglycemic rats, respectively. The maximum activity of the methanolic extract of L. micranthus (400 mg/kg) harvested from P. americana on alloxan-induced diabetic rats showed 82.59% reduction of blood sugar level at 24 h after administration determined by o-toluidine spectrophotometric method which is statistically comparable with the 83.34% of reduction for glibenclamide as a positive control. Methanolic extracts of L. micranthus from five different host trees did not show any toxicity according the acute toxicity tests in mice (LD50 values of 11650, 11650, 5900, 5900, and 5900 mg/kg for P. americana, B. nitida, K. acuminata, P. macrophylla, and A. indica, resp.). The leaves of L. micranthus parasitic on K. acuminata, A. indica, and B. nitida showed more significant antihyperglycemic activity among the other host trees investigated. The results demonstrated that the antidiabetic effect of the extract was found to be dependent on the host plant species. The weakly acidic fraction of the aqueous methanol extract of the leaves of L. micranthus parasitic on P. americana revealed anti-diabetic activity in alloxan-induced diabetic rats. The low-acidic fraction at the dose of 400 mg/kg reduced 66.60% of blood sugar level of alloxan-induced diabetic rats at 24 hours after administration . However, Osadebe and colleagues  have studied the seasonal variation for the anti-diabetic effect of the aqueous methanolic extract of the leaves of L. micranthus, parasitic on P. americana, in alloxan-induced diabetic rats. The study demonstrated that anti-diabetic effect of the extract is seasonal and dose dependent with the highest activity being at the peak of the rainy season. The results revealed 39.2% and 47.5% fasting blood sugar reduction after 6 hours consumption of 400 mg/kg of April and July samples, respectively, with 8.3% difference due to effect of seasonal variation on chemical content of leaves. Higher concentration for flavonoids in peak of the rainy season compared to the onset of rainy season could be responsible for the observed higher anti-diabetic activity in July. However, there is no data available for the particular bioactive compound(s) from L. micranthus with known mechanism for anti-diabetic activity. Therefore, this is an open area for the future research around this plant. Uzochukwu and Osadeb  have studied a comparative evaluation of antidiabetic activities of crude methanolic extract and flavonoids extract of L. micranthus harvested from K. acuminata in alloxan-induced diabetic rats. Results revealed that flavonoids extract (200 mg/kg) showed significant anti-diabetic effect within one hour of administration, while the methanolic extract (200 mg/kg) showed the significant antidiabetic effect within three hours of administration. In addition, phytoconstituents of other members of Loranthaceae plants possessing antihypertensive activity are interestingly similar to L. micranthus [48–50].
3.3. Antimicrobial Activity
Using medicinal plants as antimicrobial remedy has a long history in both developed and developing countries. In addition, in contemporary medicine because of unreasonable and indiscriminate consumption of antimicrobial drugs, the infectious microorganisms have developed resistance. Therefore, controlling the existing infectious diseases necessitates new alternative antimicrobial drug regimens . Osodebe and Ukwueze have presented the wide range of data regarding the antimicrobial activities of L. micranthus . A study on different extracts of L. micranthus leaves harvested from K. acuminata such as methanolic, ethanolic, chloroform, and petroleum ether extracts demonstrated the antibacterial activities for all tested extracts against Bacillus subtilis, Escherichia coli, and Klebsiella pneumonia, while only petroleum ether extract exerted antifungal activity against Aspergillus niger and Candida albicans with the MIC of 4.30 and 1.73 mg/mL, respectively, and no activity against Klebsiella pneumonia. The methanolic extract exhibited the most potent antibacterial effect against Bacillus subtilis and Escherichia coli with MIC of 1.58 and 1.48 mg/mL, respectively, compared to the other tested extracts . The ethyl acetate fraction of crude petroleum ether extract of L. micranthus leaves parasitic on K. acuminata also showed inhibitory activities against Candida albicans and Bacillus subtilis . A comparative investigation on antimicrobial activities of L. micranthus leaves and its phytochemicals from six different host trees, including P. americana, B. nitida, K. acuminata, P. macrophylla, A. indica, and I. gabonensis, indicated the relative significant antibacterial activities for L. micranthus parasitic on K. acuminata and P. americana. Alkaloids in these three species were found to be most abundant. Preponderance of alkaloids in these species could be responsible for the marked antimicrobial activities. The leaves extract parasitic on P. Americana exerted more potent antibacterial activity against Pseudomonas aeruginosa compared to amoxicillin, while the extracts from A. indica, P. macrophylla, and I. gabonensis exhibited stronger antibacterial activity against Staphylococcus aureus in comparison with amoxicillin . Effect of different harvesting seasons (January, April, July, and November) on antimicrobial activity of petroleum ether extracts from L. micranthus leaves, parasitic on P. americana, and its phytochemicals, also demonstrated seasonal dependency. Alkaloids as one of the major groups of antibacterial candidates was only found in July and April. Antibacterial activity against Salmonella typhi and Bacillus subtilis was markedly lower in January samples compared to the extracts from the other months’ samples [33, 54]. In addition, no significant antifungal activity for methanolic extracts of L. micranthus leaves harvested from K. acuminata, P. Americana, and I. gabonensis was demonstrated against A. niger (MIC > 3 mg/mL) and C. albicans (MIC > 4 mg/mL) compared to ketoconazole (MIC < 1 mg/mL) as an approved antifungal agent . To sum up, it has been proven that leaf extracts of L. micranthus elicited significant antibacterial activity against B. subtilis, P. aeruginosa, E. coli, and Staph. aureus without significant antifungal activity .
3.4. Antihypertensive Activity
Deaths because of hypertension arise out of cardiovascular and cerebrovascular complications including cardiac arrest, stroke, myocardial infarction, congestive heart failure, and end-stage renal disease . Early detection and commencement of treatment are vital for prevention and delaying the aftermaths and enhance the chance of longer life for afflicted patients . In the last few decades, plants have still remained as a rich source for efficacious, safe, and cost-effective antihypertensive drugs . L. micranthus is one of the plants identified with antihypertensive activity for humans and animals in sub-Saharan Africa . Aqueous extract of L. micranthus (1.32 g/kg per day) exhibited hypotensive effect on normotensive and spontaneous hypertensive rats . A noteworthy reduction in the mean arterial pressure (MAP) was obtained in both groups of rats without effect on the urinary flow rate or the respiratory rate. In addition, the level of total cholesterol exhibited significant reduction on days 6, 7, and 8 . Indeed, methanolic extract of leaves of L. micranthus demonstrated a dose-dependent inhibition of blood pressure increase in adrenaline-induced hypertensive rat. Iwalokun and colleagues  studied the activity of n-butanol, chloroform, ethyl acetate, and water fraction of the methanolic extract of L. micranthus leaf harvested from K. acuminata on pressor-induced contraction of rat aorta smooth muscles. N-Butanol fraction showed the highest dose-dependent inhibitory activity (EC50 = 0.65 mg/mL and smooth muscle relaxation of 75.2%) on rat aorta precontracted with norepinephrine and KCl, followed by decreasing order by water, chloroform, and ethyl acetate fractions. The same order of activity was observed in the ability of these fractions to reduce cardiac arginase with 11.7% reduction for n-butanol fraction and to raise serum nitric oxide with 55% elevation for n-butanol fraction in mice orally administrated 250 mg/kg of fractions for 21 days. Arginase was found to be a diagnostic indicator for cardiovascular diseases including hypertension . Enhanced activity of nitric oxide is a critical factor to reduce the vascular resistance and blood pressure that increased in hypertensive rats and humans [63, 64]. Cardiac arginase reduction, vasorelaxation, antiatherogenic events, and nitric oxide (NO) elevation were found to be responsible for antihypertensive activity of L. micranthus. Moderate and high abundance of steroids and terpenoids in n-butanol fraction strongly suggested that these phytoconstituents could be responsible for observed vasorelaxant and antiatherogenic activity of n-butanol fraction. Iwalokun and colleagues  have reviewed the possible mechanisms of antihypertensive activity of L. micranthus.
3.5. Antioxidant Activity
The role of free radicals in many diseases has been proven by recent developments in biomedical sciences. Cellular damage caused by free radicals is possibly responsible for many degenerative diseases including heart disease, cancer, brain dysfunction, and immune system decline . Phenolic compounds are potent scavengers of free radicals . The antioxidant activity of compounds 1–11 isolated from methanol extracts of L. micranthus leafy twigs was investigated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. All the tested compounds revealed significant antioxidant effect (IC50 = 23.8–50.1 μM) compared to chlorogenic acid as a positive control (IC50 = 67.9 μM). Compounds 10 and 11 exhibited the most significant antioxidant activity . The results of DPPH assay on compounds 12–14 revealed significant antioxidant potentials with EC50 of 55.42, 58.45, and 59.71 mg/mL, respectively, compared to ascorbic acid as a positive control with EC50 = 17.6 mg/mL .
3.6. Antidiarrhoeal Activity
Diarrhoea due to the risk of sever potentially fatal dehydration can be a serious complication in infants and elderly people . About 2.2 million people, mostly infants and children below 5 years, are victims of diarrhea annually . Because of the adverse effects of some existing antimotility medicines after prolonged use, many studies have been done for an alternative remedy among traditional medicinal herbs [69, 70]. In vivo study on methanolic extract of L. micranthus leaf harvested from six different host trees, including P. americana, B. nitida, K. acuminata, P. macrophylla, C. sinensis, and I. gabonensis, indicated antimotility effect in rats with castor-oil-induced diarrhoea. Methanolic extract of L. micranthus parasitic on P. macrophylla (200 mg/kg) showed the most significant decrease on defecation (93.33%) 4 hours after administration compared to atropine sulphate (2 mg/kg) as a positive control (80%). It also significantly inhibited gastrointestinal transit by 67.6% which is more than atropine sulphate (26.4%) .
3.7. Hypolipidemic Activity
The serum lipid analysis in mice orally was administered with 250 mg/kg of n-butanol, chloroform, ethyl acetate, and water fractions of the methanolic extract of L. micranthus harvested from K. acuminata for 21 days indicated with decrease in cholesterol and triglyceride with the highest activity for n-butanol fraction. Results demonstrated lower total cholesterol, triglycerides (TAG), and LDL-cholesterol levels particularly for n-butanol and water fractions after 21 days without significant changes for HDL-cholesterol. On day 21, n-butanol fraction significantly reduced TAG level ( versus mg/dL). Total cholesterol and LDL-cholesterol levels also reduced from mg/dL to mg/dL and from mg/dL to mg/dL by n-butanol fraction on the day 21, respectively .
The overview of scientific investigations on L. micranthus showed various biological activities for this plant. The phytochemical constituents and the activity of the medicinal values of L. micranthus are strongly dependent on harvesting season and host species trees. Phytochemicals and compounds isolated from L. micranthus are in interest for the further investigations towards application of this plant as anti-diabetic, antibacterial, antihypertensive, hypolipidemic, antioxidant, antidiarrhoeal, and immunomodulatory medicines. Further in vivo genotoxic tests of this plant can be also beneficial for the safety approval for therapeutic applications.
Conflict of Interests
All authors declared that there is no actual or potential conflict of interests including any financial, personal, or other relationships with other people or organizations that could inappropriately influence or be perceived to influence their work.
The authors thank the Ministry of Higher Education (MOHE), Malaysia, for High Impact Research (HIR) MOHE Grant (E000013-20001). They also would like to thank the University of Malaya for University Malaya Research Grant (UMRG) (RG383-11HTM).
- P. Griggs, “Mistletoe, myth, magic and medicine,” The Biochemist, vol. 13, pp. 3–4, 1991.
- N. Nwude and M. A. Ibrahim, “Plants used in traditional veterinary medical practice in Nigeria,” Journal of Veterinary Pharmacology and Therapeutics, vol. 3, no. 4, pp. 261–273, 1980.
- E. Kafaru, “‘Mistletoe—an example of an all-purpose herb’ Herbal Remedies,” Guardian Newspaper, vol. 3, p. 11, 1993.
- T. Fukunaga, I. Kajikawa, K. Nishiya, K. Takeya, and H. Itokawa, “Studies on the constituents of Japanese mistletoes from different host trees, and their antimicrobial and hypotensive properties,” Chemical and Pharmaceutical Bulletin, vol. 37, no. 6, pp. 1543–1546, 1989.
- R. Scheer, A. Scheffler, and M. Errenst, “Two harvesting times, summer and winter: are they essential for preparing pharmaceuticals from mistletoe (Viscum album)?” Planta Medica, vol. 58, no. 7, pp. 594–599, 1992.
- D. K. Obatomi, E. O. Bikomo, and V. J. Temple, “Anti-diabetic properties of the African mistletoe in streptozotocin-induced diabetic rats,” Journal of Ethnopharmacology, vol. 43, no. 1, pp. 13–17, 1994.
- M. L. Wagner, T. Fernandez, E. Alvarez, R. A. Ricco, S. Hajos, and A. A. Gurni, “Micromolecular and macromolecular comparison of Argentina mistletoe (Ligaria cuneifolia) and European mistletoe (Viscum album L.),” Acta Farmaceutica Bonaerense, vol. 15, no. 2, pp. 99–108, 1996.
- F. H. Ali, T. N. Intesar, W. A. Khylood, and A. H. Saad, “Hematopoietic toxicity of Loranthus europaeus chloroform extract: in vitro study,” International Journal of Comprehensive Pharmacy, vol. 17, pp. 345–352, 2005.
- P. O. Osadebe, C. C. Abba, and M. O. Agbo, “Antimotility effects of extracts and fractions of Eastern Nigeria mistletoe (Loranthus micranthus Linn),” Asian Pacific Journal of Tropical Medicine, vol. 5, no. 7, pp. 556–560, 2012.
- P. O. Osadebe and E. O. Omeje, “Comparative acute toxicities and immunomodulatory potentials of five Eastern Nigeria mistletoes,” Journal of Ethnopharmacology, vol. 126, no. 2, pp. 287–293, 2009.
- B. Oliver-Bever, Medicinal Plants in Tropical West Africa, Cambridge University Press, New York, NY, USA, 1986.
- M. O. Agbo, D. Lai, F. B. C. Okoye, P. O. Osadebe, and P. Proksch, “Antioxidative polyphenols from Nigerian mistletoe Loranthus micranthus (Linn.) parasitizing on Hevea brasiliensis,” Fitoterapia, vol. 86, pp. 78–83, 2013.
- A. L. Davis, Y. Cai, A. P. Davies, and J. R. Lewis, “1H and 13C NMR assignments of some green tea polyphenols,” Magnetic Resonance in Chemistry, vol. 34, no. 11, pp. 887–890, 1996.
- P. Fan, H. Lou, W. Yu, D. Ren, B. Ma, and M. Ji, “Novel flavanol derivatives from grape seeds,” Tetrahedron Letters, vol. 45, no. 15, pp. 3163–3166, 2004.
- A. Gramza and J. Korczak, “Tea constituents (Camellia sinensis L.) as antioxidants in lipid systems,” Trends in Food Science and Technology, vol. 16, no. 8, pp. 351–358, 2005.
- M. Singh, M. Arseneault, T. Sanderson, V. Murthy, and C. Ramassamy, “Challenges for research on polyphenols from foods in Alzheimer's disease: bioavailability, metabolism, and cellular and molecular mechanisms,” Journal of Agricultural and Food Chemistry, vol. 56, no. 13, pp. 4855–4873, 2008.
- M. M. Manir, J. K. Kim, B.-G. Lee, and S.-S. Moon, “Tea catechins and flavonoids from the leaves of Camellia sinensis inhibit yeast alcohol dehydrogenase,” Bioorganic and Medicinal Chemistry, vol. 20, no. 7, pp. 2376–2381, 2012.
- L. Sánchez-del-Campo, F. Otón, A. Tárraga, J. Cabezas-Herrera, S. Chazarra, and J. N. Rodríguez-López, “Synthesis and biological activity of a 3,4,5-trimethoxybenzoyl ester analogue of epicatechin-3-gallate,” Journal of Medicinal Chemistry, vol. 51, no. 7, pp. 2018–2026, 2008.
- O. A. Eldahshan, “Isolation and structure elucidation of phenolic compounds of carob leaves grown in Egypt,” Current Research Journal of Biological Sciences, vol. 3, pp. 53–55, 2011.
- M. Materska and I. Perucka, “Antioxidant activity of the main phenolic compounds isolated from hot pepper fruit (Capsicum annuum L.),” Journal of Agricultural and Food Chemistry, vol. 53, no. 5, pp. 1750–1756, 2005.
- E. N. Frankel, A. L. Waterhouse, and P. L. Teissedre, “Principal phenolic phytochemicals in selected California wines and their antioxidant activity in inhibiting oxidation of human low-density lipoproteins,” Journal of Agricultural and Food Chemistry, vol. 43, no. 4, pp. 890–894, 1995.
- E. O. Omeje, O. P. Ogoamaka, A. Kawamura et al., “Three—(−) catechin-o-rhamnosides from the Eastern Nigeria mistletoe with potent immunostimulatory and antioxidant activities,” Biomolecules, vol. 1, no. 1, pp. 1–6, 2012.
- E. O. Omeje, P. O. Osadebe, C. O. Esimone, C. S. Nworu, A. Kawamura, and P. Proksch, “Three hydroxylatedlupeol-based triterpenoid esters isolated from the Eastern Nigeria mistletoe parasitic on Kola acuminata,” Natural Product Research, vol. 26, no. 19, pp. 1775–1781, 2012.
- O. E. Ogechukwu, O. P. Ogoamaka, N. C. Sylvester et al., “Steroids and triterpenoids from Eastern Nigeria mistletoe, Loranthus micranthus Linn. (Loranthaceae) parasitic on Kola acuminata with immunomodulatory potentials,” Phytochemistry Letters, vol. 4, no. 3, pp. 357–362, 2011.
- P. Osadebe, E. Omeje, A. Kawamura, and F. Okoye, “Lupeol derivatives from the Eastern Nigeria mistletoe, Loranthus micranthus Linn. (Loranthaceae) with enhanced cell proliferative potentials,” Planta Medica, vol. 76, article 091, 2010.
- E. Omeje, P. Osadebe, P. Procksh et al., “Immunomodulatory and antioxidant constituents of Eastern Nigeria mistletoe, Loranthus micranthus Linn. (Loranthaceae) parasitic on Cola acuminata Schott et Endl.,” Planta Medica, vol. 76, p. 129, 2010.
- E. O. Omeje, P. O. Osadebe, C. S. Nworu et al., “A novel sesquiterpene acid and an alkaloid from leaves of the Eastern Nigeria mistletoe, Loranthus micranthus with potent immunostimulatory activity on C57BL6 mice splenocytes and CD69 molecule,” Pharmaceutical Biology, vol. 49, no. 12, pp. 1271–1276, 2011.
- P. O. Osadebe, E. O. Omeje, S. C. Nworu et al., “Antidiabetic principles of Loranthus micranthus Linn. parasitic on Persea americana,” Asian Pacific Journal of Tropical Medicine, vol. 3, no. 8, pp. 619–623, 2010.
- P. O. Osadebe, E. O. Omeje, P. F. Uzor, E. K. David, and D. C. Obiorah, “Seasonal variation for the antidiabetic activity of Loranthus micranthus methanol extract,” Asian Pacific Journal of Tropical Medicine, vol. 3, no. 3, pp. 196–199, 2010.
- B. A. Iwalokun, S. A. Hodonu, S. Nwoke, O. Ojo, and P. U. Agomo, “Evaluation of the possible mechanisms of antihypertensive activity of Loranthus micranthus: an african mistletoe,” Biochemistry Research International, vol. 2011, Article ID 159439, 9 pages, 2011.
- P. O. Osadebe and S. E. Ukwueze, “A comparative study of the phytochemical and anti-microbial properties of the Eastern Nigerian specie of African Mistletoe (Loranthus micranthus) sourced from different host trees,” Bio-Research, vol. 2, no. 1, pp. 18–23, 2004.
- P. O. Osadebe, G. B. Okide, and I. C. Akabogu, “Study on anti-diabetic activities of crude methanolic extracts of Loranthus micranthus (Linn.) sourced from five different host trees,” Journal of Ethnopharmacology, vol. 95, no. 2-3, pp. 133–138, 2004.
- P. O. Osadebe, C. A. Dieke, and F. B. C. Okoye, “A study of the seasonal variation in the antimicrobial constituents of the leaves of Loranthus micranthus sourced from Percia americana,” Planta Medica, vol. 73, pp. 205–210, 2007.
- L. M. Perry and J. Metzger, Medicinal Plants of East and South East Asia: Attributed Properties and Uses, MIT Press, Cambridge, Mass, USA, 1980.
- D. O. Edem and I. F. Usoh, “Biochemical changes in Wistar rats on oral doses of mistletoe (Loranthus micranthus),” American Journal of Pharmacology and Toxicology, vol. 4, no. 3, pp. 94–97, 2009.
- B. A. Iwalokun, A. O. Oyenuga, G. M. Saibu GM, and J. Ayorinde, “Analyses of cytotoxic and genotoxic potentials of Loranthus micranthus using the Allium cepa test,” Current Research Journal of Biological Sciences, vol. 3, no. 5, pp. 459–467, 2011.
- H. M. Kim, S. B. Han, G. T. Oh et al., “Stimulation of humoral and cell mediated immunity by polysaccharide from mushroom Phellinus linteus,” International Journal of Immunopharmacology, vol. 18, no. 5, pp. 295–303, 1996.
- C. K. Ameho, A. A. Adjei, K. Yamauchi et al., “Modulation of age-related changes in immune functions of protein-deficient senescence-accelerated mice by dietary nucleoside-nucleotide mixture supplementation,” British Journal of Nutrition, vol. 77, no. 5, pp. 795–804, 1997.
- L. A. Ande, “Mistletoe: the magic herb,” Pharmacology Journal, vol. 229, pp. 437–439, 1982.
- E. Juanita, “Mistletoe: good for more than free kisses,” Journal of American Botanical Council, vol. 68, pp. 50–59, 2005.
- P. O. Osadebe and E. O. Omeje, “Isolation and characterisation of antiviral and immunomodulatory constituents from Nigerian mistletoe, Loranthus micranthus,” Planta Medica, vol. 73, p. 108, 2007.
- P. O. Osadebe and E. O. Omeje, “Immunomodulatory activities of n-hexane and methanol extracts of Loranthus micranthus Linn. parasitic on Parkia biglobosa,” Planta Medica, vol. 74, article 217, 2008.
- P. O. Osadebe and E. O. Omeje, “Preliminary fractionation indicates that flavonoids, steroids and terpenoids are the main immunomodulatory constituents of Loranthus micranthus (Linn),” Planta Medica, vol. 75, article 78, 2009.
- O. E. Ogechukwu, O. P. Ogoamaka, N. C. Sylvester, A. Kawamura, and P. Proksch, “Immunomodulatory activity of a lupane triterpenoid ester isolated from the eastern Nigeria mistletoe, Loranthus micranthus (Linn),” Asian Pacific Journal of Tropical Medicine, vol. 4, no. 7, pp. 514–522, 2011.
- S. H. Lee and Y. J. Jeon, “Anti-diabetic effects of brown algae derived phlorotannins, marine polyphenols through diverse mechanisms,” Fitoterapia, vol. 86, pp. 129–136, 2013.
- R. A. DeFronzo, “Pharmacologic therapy for type 2 diabetes mellitus,” Annals of Internal Medicine, vol. 131, no. 4, pp. 281–303, 1999.
- I. C. Uzochukwu and P. O. Osadeb, “Comparative evaluation of antidiabetic activities of flavonoids extract and crude methanol extract of Loranthus micranthus parasitic on Kola acuminata,” Journal of Pharmaceutical and Allied Sciences, vol. 4, no. 1, pp. 2–7, 2007.
- Z. J. Wang, Z. Q. Yang, T. N. Huang, L. Wen, and Y. W. Liu, “Experimental research on inhibitory effect of alcohol extracts from Loranthus yadoriki Sieb. on coxsackie B3 virus,” Zhongguo Zhong Yao Za Zhi, vol. 25, no. 11, pp. 685–687, 2000.
- Y.-K. Kim, S. K. Young, U. C. Sang, and Y. R. Shi, “Isolation of flavonol rhamnosides from Loranthus tanakae and cytotoxic effect of them on human tumor cell lines,” Archives of Pharmacal Research, vol. 27, no. 1, pp. 44–47, 2004.
- O. Z. Ameer, I. M. Salman, M. J. A. Siddiqui et al., “Characterization of the possible mechanisms underlying the hypotensive and spasmogenic effects of Loranthus ferrugineus methanolic extract,” American Journal of Chinese Medicine, vol. 37, no. 5, pp. 991–1008, 2009.
- P. Abirami, M. Gomathinayagam, and R. Panneerselvam, “Preliminary study on the antimicrobial activity of Enicostemma littorale using different solvents,” Asian Pacific Journal of Tropical Medicine, vol. 5, no. 7, pp. 552–555, 2012.
- P. O. Osadebe and I. C. Akabogu, “Antimicrobial activity of Loranthus micranthus harvested from kola nut tree,” Fitoterapia, vol. 77, no. 1, pp. 54–56, 2006.
- E. A. C. Cemaluk and N. N. Emeka, “Phytochemical properties of some solvent fractions of petroleum ether extract of the African mistletoe (Loranthus micranthus Linn) leaves and their antimicrobial activity,” African Journal of Biotechnology, vol. 11, no. 62, pp. 12595–12599, 2012.
- P. O. Osadebe, C. A. Dieke, and F. B. C. Okoye, “A study of the seasonal variation in the antimicrobial constituents of the leaves of Loranthus micranthus sourced from Percia americana,” Research Journal of Medicinal Plant, vol. 2, no. 1, pp. 48–52, 2008.
- S. E. Ukwueze and P. O. Osadebe, “Determination of anti-fungal properties of the african mistletoe species: Loranthus micranthus L.,” International Journal of Pharma and Bio Sciences, vol. 3, no. 1, pp. 454–458, 2012.
- S. E. Ukwueze, P. O. Osadebe, and N. O. Ezenobi, “Bioassay-guided evaluation of the antibacterial activity of Loranthus species of the African mistletoe,” International Journal of Pharmaceutical and Biomedical Research, vol. 4, no. 2, pp. 79–82, 2013.
- J. Fry, “Deaths and complications from hypertension,” Journal of the Royal College of General Practitioners, vol. 25, no. 156, pp. 489–494, 1975.
- P. A. Meredith, “Candesartan cilexetil—a review of effects on cardiovascular complications in hypertension and chronic heart failure,” Current Medical Research and Opinion, vol. 23, no. 7, pp. 1693–1705, 2007.
- G. Y. Yeh, D. M. Eisenberg, T. J. Kaptchuk, and R. S. Phillips, “Systematic review of herbs and dietary supplements for glycemic control in diabetes,” Diabetes Care, vol. 26, no. 4, pp. 1277–1294, 2003.
- O. C. Amira and N. U. Okubadejo, “Frequency of complementary and alternative medicine utilization in hypertensive patients attending an urban tertiary care centre in Nigeria,” BMC Complementary and Alternative Medicine, vol. 7, article 30, 2007.
- D. K. Obatomi, V. O. Aina, and V. J. Temple, “Effects of African mistletoe extract on blood pressure in spontaneously hypertensive rats,” Pharmaceutical Biology, vol. 34, no. 2, pp. 124–127, 1996.
- C. Demougeot, A. Prigent-Tessier, C. Marie, and A. Berthelot, “Arginase inhibition reduces endothelial dysfunction and blood pressure rising in spontaneously hypertensive rats,” Journal of Hypertension, vol. 23, no. 5, pp. 971–978, 2005.
- J. G. Umans and R. Levi, “Nitric oxide in the regulation of blood flow and arterial pressure,” Annual Review of Physiology, vol. 57, pp. 771–790, 1995.
- C.-C. Wu and M.-H. Yen, “Higher level of plasma nitric oxide in spontaneously hypertensive rats,” American Journal of Hypertension, vol. 12, no. 5, pp. 476–482, 1999.
- O. I. Aruoma, “Free radicals, oxidative stress, and antioxidants in human health and disease,” Journal of the American Oil Chemists' Society, vol. 75, no. 2, pp. 199–212, 1998.
- M. K. Zainol, A. Abd-Hamid, S. Yusof, and R. Muse, “Antioxidative activity and total phenolic compounds of leaf, root and petiole of four accessions of Centella asiatica (L.) Urban,” Food Chemistry, vol. 81, no. 4, pp. 575–581, 2003.
- G. F. Longstreth, W. G. Thompson, W. D. Chey, L. A. Houghton, F. Mearin, and R. C. Spiller, “Functional bowel disorder,” Gastroenterology, vol. 130, no. 5, pp. 1480–1491, 2006.
- N. Venkatesan, V. Thiyagarajan, S. Narayanan et al., “Anti-diarrhoeal potential of Asparagus racemosus wild root extracts in laboratory animals,” Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 1, pp. 39–45, 2005.
- J. D. Gale, “The use of novel promotility and prosecretory agents for the treatment of chronic idiopathic constipation and irritable bowel syndrome with constipation,” Advances in Therapy, vol. 26, no. 5, pp. 519–530, 2009.
- S. Rajan, H. Suganya, T. Thirunalasundari, and S. Jeeva, “Antidiarrhoeal efficacy of Mangifera indica seed kernel on Swiss albino mice,” Asian Pacific Journal of Tropical Medicine, vol. 5, no. 8, pp. 630–633, 2012.