Figure 4: Vitexicarpin induces apoptosis of VEGF-stimulated endothelial cells. (a) Vitexicarpin increases annexin V (+) cells at indicted concentrations. (b) Hoechst 33342 staining of apoptotic endothelial cells. Cells are magnified in inset figure. (c) DNA fragment in endothelial cells treated with vitexicarpin at indicted concentrations for 12 h. (d) Effects of vitexicarpin on pro-casp-3, pro-casp-7, PARP, and cleaved-PARP. β-actin is used as a loading control. ; compared with VEGF group.