A Small Molecule Swertisin from Enicostemma littorale Differentiates NIH3T3 Cells into Islet-Like Clusters and Restores Normoglycemia upon Transplantation in Diabetic Balb/c Mice
Table 2
List of primer sequences of RT-PCR with annealing temperature and amplicon size.
Name of gene
Gene accession number
Primer sequence forward
Primer sequence reverse
PCR conditions (Tm)
Amplicon size (BP)
α-SMA
NT_039687.7
AGTCGCCATCAGGAACCTCGAG
ATCTTTTCCATGTCGTCCCAGTTG
60
296
Vimentin
NT_039202.7
AGCGGGACAACCTGGCCG
GGGAAGAAAAGGTTGGCAGAGGC
58
744
Nestin
NT_039240.7
GCGGGGCGGTGCGTGACTAC
AGGCAAGGGGGAAGAGAAGGATGT
58
326
PDX-1
NT_039324.7
CTC GCT GGG AAC GCT GGA ACA
GCT TTG GTG GAT TTC ATC CAC GG
55
229
Ngn-3
NT_039500.7
ACTAGGATGGCGCCTCATCCCTTG
GGTCTCTTCACAAGAAGTCTGAGA
57
658
Pax-4
NM_011038.2
TGGCTTCCTGTCCTTCTGTGAGG
TCCAAGACTCCTGTGCGGTAGTAG
62
214
Nkx-6.1
NM_144955.2
ATGGGAAGAGAAAACACACCAGAC
TAATCGTCGTCGTCCTCCTCGTTC
60
280
Insulin
NM_008386.3
GCCCAGGCTTTTGTCAAACA
CTCCCCACACACCAGGTAGAG
55
90
Glucagon
NM_008100.3
ATGAAGACCATTTACTTTGTGGCT
GGTGTTCATCAACCACTGCAC
58
243
Reg-1
NM_009042.1
AAGCTGAAGAAGACCTGCCA
TGTTAGGAGACCCAGTTGCC
60
123
GAPDH
NT_166349.1
CAAGGTCATCCATGACAACTTTG
GTCCACCACCCTGTTGCTGTAG
58
496
List of primer sequences for Q-PCR with annealing temperature and amplicon size