Ellagic Acid Reduces Adipogenesis through Inhibition of Differentiation-Prevention of the Induction of Rb Phosphorylation in 3T3-L1 Adipocytes
Figure 4
EA inhibits the cell number increase at early clonal expansion phase of adipogenesis. 3T3-L1 preadipocytes were induced to differentiate in the presence of control medium or EA for the indicated times. (a) On indicated days, cells were trypsinized and enumerated. (b) Cells were treated for 48 h at 37°C in 5 mL culture medium containing EA at final concentrations of 0 and 20 μM. Then cells were stained with PI and Annexin V-FITC and assessed by FACScan flow cytometer, the apoptotic rate of cell compared with control group (without EA). (c and d) DNA content was analyzed by PI staining and flow cytometry at given time points and analyzed. (e) At 16, 20, and 24 hrs after induction, cells were labeled with EdU for 2 hrs and then stained with Hoechst. The fluorescence of EdU (red) and Hoechst (blue) was detected with a fluorescence microscope. Results are the mean ± SD of 3 independent experiments, each performed in triplicate.
