Ellagic Acid Reduces Adipogenesis through Inhibition of Differentiation-Prevention of the Induction of Rb Phosphorylation in 3T3-L1 Adipocytes
Figure 5
EA reduces Cyclin A protein expression and Rb phosphorylation. 3T3-L1 preadipocytes were induced to differentiate in the presence of control medium (c) or EA (e) for 16, 20, or 24 h, as indicated. Solubilised protein from duplicate cultures was immunoblotted with antibodies against Cyclin A, phospho-Rb, Rb, or β-actin (loading control). Immunoblots shown are representative of 3 independent experiments (a and c). Densitometric data are expressed as mean ± SD of 3 independent experiments, each performed in duplicate (b and d). compared to control (without EA treatment).
