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Evidence-Based Complementary and Alternative Medicine
Volume 2013 (2013), Article ID 291592, 9 pages
http://dx.doi.org/10.1155/2013/291592
Research Article

Efficacy of Plectranthus amboinicus (Lour.) Spreng in a Murine Model of Methicillin-Resistant Staphylococcus aureus Skin Abscesses

1Department of Clinical Analysis and Toxicology, Faculty of Pharmacy, Universidade Federal do Ceará (UFC), Rua Capitão Francisco Pedro 1210, 60430-370 Fortaleza, CE, Brazil
2Department of Pharmacy, Faculty of Pharmacy, UFC, Rua Capitão Francisco Pedro 1210, 60430-370 Fortaleza, CE, Brazil
3Department of Pathology and Legal Medicine, Faculty of Medicine, UFC, Rua Monsenhor Furtado S/N, 60430-350 Fortaleza, CE, Brazil

Received 17 September 2012; Accepted 17 January 2013

Academic Editor: Ulysses Paulino de Albuquerque

Copyright © 2013 Francisco Fábio Martins de Oliveira et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The present work aimed to evaluate the effectiveness of Plectranthus amboinicus (Lour.) Spreng against MRSA clinical isolates. The in vitro antimicrobial activity of the hydroalcoholic extract (HE), the ethyl acetate (EA) fraction and its subfractions were determined by broth microdilution and bioautography against MRSA clinical isolates. The microdilution checkerboard method was used to assess in vitro drug combination studies. To induce abscess formation, bacterial suspensions were added to Citodex and inoculated subcutaneously into male Swiss mice. The treatment protocol consisted of 2 doses of HE, the EA fraction or vancomycin introduced intraperitoneally into mice 3 and 12 h after infection. The EA fraction and its subfractions presented the lowest minimal inhibitory concentrations (MIC, 0.25 to 0.5 mg/mL). The plant samples were bacteriostatic at 2x and 4x MIC and bactericidal at 100 mg/mL. The EA fraction presented synergism with vancomycin and an additive effect with ciprofloxacin. A significant reduction of abscess volume, bacterial cell counts in abscess slurries, and inflammatory scores was observed in the HE and EA fraction-treated groups. The samples were effective in treating the animals in a dose-dependent fashion. The present study proved the effectiveness of P. amboinicus fractions against MRSA using in vitro and in vivo assays.