Prenylated Flavonoids from Morus alba L. Cause Inhibition of G1/S Transition in THP-1 Human Leukemia Cells and Prevent the Lipopolysaccharide-Induced Inflammatory Response
Figure 5
Effects of M. alba L. prenylated flavonoids and the reference drug parthenolide (PTL) on LPS-induced (pro-)MMP-2 activity at SCp2 cells. Cells were pretreated with given compounds (5 μM), parthenolide (5 μM), or the vehicle (DMSO) only. After 1 h of incubation, the inflammatory response was induced by LPS (except for the control cells). Activity of (pro-)MMP-2 was detected by zymography (a). Intensity of digested bands was analyzed by densitometry. (b) shows pro-MMP-2/MMP-2 ratio. Showed gels represent results of three independent experiments (c). Results are expressed as means ± S.E. for three independent experiments. ***Significant difference in comparison to vehicle-treated cells (), #significant difference in comparison to parthenolide-treated cells (), and ##significant difference in comparison to parthenolide-treated cells ().
