Microarray Analysis of mRNA and MicroRNA Expression Profile Reveals the Role of β-Sitosterol-D-glucoside in the Proliferation of Neural Stem Cell

Table 2

RT Primer sequence of the internal control gene and the target genes for cDNA synthesis.

Genes	RT primer sequence
U6	5′-CGCTTCACGAATTTGCGTGTCAT-3′
rno-miR-129-5p	5′-GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACGCAAGCC-3′
rno-miR-322-5p	5′-GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTCCAAAA-3′
rno-miR-301a-3p	5′-GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACGCTTTG-3′

To validate the miRNA expression profiling, three genes were selected to performed real time-PCR. RT primer sequences of the U6 gene used as reference and the genes selected were listed in Table 2.