Microarray Analysis of mRNA and MicroRNA Expression Profile Reveals the Role of β-Sitosterol-D-glucoside in the Proliferation of Neural Stem Cell

Table 4

Upregulation genes.

GO ID	Term	𝑃 value	Genes
GO:0000087	M phase of the cell cycle	9.58𝐸−09	AURKB; CCNF; KIF2C; BUB1B; CDCA3; IGF1; CENPF; DLGAP5; CDC20; SPAG5; MAD2L1; TRAF4AF1; NUSAP1; ESPL1; CCNB1; PLK1; PTTG1
GO:0000278	Mitotic cell cycle	8.23𝐸−08	SPAG5; MAD2L1; TRAF4AF1; NUSAP1; ESPL1; CDKN1B; CENPF; DLGAP5; CENPA; NDC80; CCNB1; PTTG1; CDC20; AURKB; CCNF;
GO:0000280	Nuclear division	1.82𝐸−07	SPAG5; MAD2L1; TRAF4AF1; NUSAP1; ESPL1; CCNB1; PLK1; PTTG1; CDC20; AURKB; CCNF; KIF2C; BUB1B; CDCA3; IGF1
GO:0007059	Chromosome segregation	4.12𝐸−07	SPAG5; MAD2L1; TRAF4AF1; NUSAP1; ESPL1; CCNB1; NDC80; PTTG1; CENPF; KIF2C; TOP2A
GO:0048285	Organelle fission	4.86𝐸−07	SPAG5; MAD2L1; TRAF4AF1; NUSAP1; ESPL1; CCNB1; CDCA3; PLK1; PTTG1; CDC20; AURKB; CCNF; KIF2C; BUB1B; IGF1
GO:0000226	Microtubule cytoskeleton organization	1.87𝐸−06	CENPA; NDC80; ESPL1; PLK1; KIF20A; KIF2C; SPAG5; TEKT1; TRAF4AF1; CCNF; TACC3; CDKN1B; NUSAP1; CCNB1; AURKB
GO:0048545	Response to steroid hormone stimulus	2.36𝐸−06	CAR9; GBA; A2M; HP; IGF1; ADM; and so forth, a total of 22 genes
GO:0051301	Cell division	3.80𝐸−06	NUSAP1; PLK1; AURKB; KIF20A; NUMBL; TOP2A; TXNIP; CCNB2; CCNB1; PTTG1; CDC20; CCNF; KIF2C; BUB1B; CDCA3; TRAF4AF1
GO:0008283	Cell proliferation	6.93𝐸−06	CDC20; AURKB; CCNB1; PTTG1; IGF1; and so forth, a total of 39 genes
GO:0019932	Second messenger-mediated signaling	1.12𝐸−05	CALCA; ADORA2A; GRM3; EDNRB; GRM5; CXCR4; TOX3; RASD1; ADM; PDE7B; CDH13; IGF1; LMCD1; MT1A
GO:0009605	Response to external stimulus	1.25𝐸−05	A2M; LBP; CCNB1; ENPP2; CKLF; IGF1; and so forth, a total of 35 genes
GO:0009719	Response to endogenous stimulus	1.94𝐸−05	ADORA2A; IGF1; SPP1; A2M; and so forth, a total of 32 genes
GO:0008608	Attachment of spindle microtubules to kinetochores	2.25𝐸−05	CCNB1; SPAG5; TRAF4AF1; NDC80
GO:0009056	Catabolism	2.78𝐸−05	TOP2A; FBXO32; MANBA; IGF1; CDC20; and so forth, totle 41 genes
GO:0007051	Spindle organization	3.11𝐸−05	ESPL1; NDC80; TACC3; CCNB1; AURKB; SPAG5; TRAF4AF1
GO:0051313	Attachment of spindle microtubules to chromosomes	4.44𝐸−05	NDC80; CCNB1; SPAG5; TRAF4AF1
GO:0009725	Response to hormone stimulus	5.00𝐸−05	IGF1; LOX; SPP1; A2M; HP; ALPL; and so forth, a total of 27 genes
GO:0010941	Regulation of cell death	2.39𝐸−04	AURKB; IGF1; ADORA2A; and so forth, a total of 32 genes
GO:0043470	Regulation of carbohydrate catabolism	8.83𝐸−04	PFKFB3; DDIT4; IER3; IGF1
GO:0048016	Inositol phosphate-mediated signaling	1.28𝐸−03	EDNRB; GRM5; CALCA; IGF1; LMCD1

Upregulation genes obtained from the mRNA expression profiling were listed in Table 4. The upregulation genes were mostly involved in the mitotic cell cycle, enhancing cell proliferation. 𝑃 value, the significance testing value of the GO ID, results from the top GO of a bioconductor.