Microarray Analysis of mRNA and MicroRNA Expression Profile Reveals the Role of β-Sitosterol-D-glucoside in the Proliferation of Neural Stem Cell

Table 5

Downregulation genes.

GO ID	Term	𝑃 value	Genes
GO:0030154	Cell differentiation	3.02𝐸−06	FOXC2; IGF2; JAG1; SEMA3C; HMGA2; and so forth, a total of 38 genes
GO:0006950	Response to stress	2.05𝐸−04	PENK; BDNF; CRYAB; TRH; PLAU; MMP3; and so forth, a total of 32 genes
GO:2000736	Regulation of stem cell differentiation	4.89𝐸−04	HMGA2; JAG1; HES1
GO:0032103	Positive regulation of response to external stimulus	6.01𝐸−04	NPY; IL1RL1; SCG2; TNFSF11; CD74; THBS4
GO:0048710	Regulation of astrocyte differentiation	8.72𝐸−04	HES1; CLCF1; HMGA2
GO:0045597	Positive regulation of cell differentiation	1.16𝐸−03	TGFB1I1; CD74; FRZB; JAG1; BDNF; MAP1B; IFI204; TNFSF11; TNFRSF12A; HES1; CLCF1
GO:0048584	Positive regulation of response to stimulus	2.06𝐸−03	CD74; TNFSF11; TGFB1I1; CDKN1C; GPC3; HES1; NPY; CLCF1; IGF2; JAG1; PRRX2; IL1RL1; SCG2; THBS4; HMGA2; TNFRSF12A
GO:0000904	Cell morphogenesis involved in differentiation	5.26𝐸−03	HMGA2; MAP1B; BDNF; TGFB1I1; CHST3; FOXC2; TNFRSF12A; HES1; XYLT1; NPTX1
GO:0050920	Regulation of chemotaxis	6.38𝐸−03	SCG2; CD74; THBS4; EFNB2
GO:0030182	Neuron differentiation	7.29𝐸−03	MAP1B; BDNF; CHST3; NPY; MFRP; JAG1; HES1; CDKN1C; TNFRSF12A; HCN1; XYLT1; THBS4; NPTX1; BYSL
GO:0060326	Cell chemotaxis	1.53𝐸−02	TNFSF11; SCG2; CD74; THBS4
GO:0090398	Cellular senescence	1.67𝐸−02	HMGA2; RGD1305645
GO:0016477	Cell migration	2.55𝐸−02	SEMA3C; EFNB2; TNFSF11; TNFRSF12A; PLAU; MMP3; UNC5C; SCG2; THBS4; CD74; HES1
GO:0016126	Sterol biosynthesis	4.53𝐸−02	HMGCS2; HSD17B7
GO:0033554	Cellular response to stress	4.91𝐸−02	HMGA2; TNFSF11; MAP1B; CHST3; XYLT1; DHX9

Downregulation genes obtained from the mRNA expression profiling were listed in Table 5. The downregulation genes were mostly involved in differentiation and the regulation of differentiation, indicating that cell differentiation was inhibited, and accordingly, more possibility of cell proliferation was afforded. 𝑃 value, the significance testing value of the GO ID, results from the top GO of a bioconductor.