364519.fig.001a
(a)
364519.fig.001b
(b)
Figure 1: Hydrogen peroxide treatment induces tyrosine phosphorylation in H9C2 cells. (a) Total cell lysates were prepared from H9C2 cells treated with a range of H2O2 concentrations (0, 0.2, 0.5, 1, 5, and 10 mM) for 20 min. H9C2 total cell lysate proteins were separated by 1D SDS-PAGE, transferred onto a PVDF membrane (Pall) electrophoretically, and then probed with specific primary antibodies antiphosphotyrosine and β-actin. (b) Effects of quercetin on H2O2-induced tyrosine phosphorylation in H9C2 cells. The total cell lysates were prepared from H9C2 cells pretreated with different quercetin concentrations (0, 0.2, 0.5, 0.7, 1, and 10 mM) for 1 h and then treated with 5 mM H2O2 for 20 min. Cells were immunoblotted with phosphotyrosine and β-actin (upper image). β-Actin is a loading control for this experiment.