Table 1: Differentially expressed proteins were listed alphabetically after 2D-DIGE and MALDI-TOF mass spectrometry analysis in H9C2 cells in response to H2O2 treated and pretreated with quercetin. The average ratios of these 44 spots are differentially expressed between untreated (control), H2O2-treated, and quercetin-pretreated followed by treatment with H2O2, calculated from triplicate gels. Boldface numbers represent proteins for which the changes between the H2O2 treatment and the control are significantly greater than changes between quercetin pretreatment followed by H2O2 treatment and control.

Spot no.Swissprot no.Protein namePred. MWPred. PICov. %MASCOT scoreNo. of peptides
match/
supplied
H2O2/CtrlQuercetin + H2O2/CtrlPeptide sequenceFunction

1420P6332440S ribosomal protein S12148586.8242%55/516/30−1.25−2.77QAHLCVLASNCDEPMYVK,
TALIHDGLAR
Protein synthesis
691Q9JLJ34-Trimethylaminobutyraldehyde dehydrogenase545306.5744%109/5616/661.681.9AFEPATGR,
AGAPNGLFNVVQGGAATGQFLCQHR
Protein synthesis
473P6303960 kDa heat shock protein (mitochondrial)610885.9120%61/568/301.371.54AAVEEGIVLGGGCALLR,
ISSVQSIVPALEIANAHR
Chaperon
1010P60711Actin (cytoplasmic)420525.2923%54/516/37−1.51−1.27GYSFTTTAER,
SYELPDGQVITIGNER
Cytoskeleton
704P11884Aldehyde dehydrogenase, mitochondria569666.6341%143/5616/541.121.56RVTLELGGK,
SGQQEGAK
Redox regulation
990P54921Alpha-soluble NSF attachment protein336275.350%120/5613/399.857.69QAEAMALLAEAER,
IEEACEIYAR
Transport
1002P55260Annexin A4361685.3139%145/5113/28−1.92−2.35GDTSGDYR,
WGTDEVK
Transport/Ca2+
396P48037Annexin A6761065.3952%253/5636/74−1.35−1.73YELTGKFER,
AINEAYKEDYHK
Transport/Ca2+
1116P35426Cell division protein kinase 4340066.0954%198/5615/323.53.22VTLVFEHIDQDLR,
VPNGGAAGGGLPVSTVR
Cell cycle
1319P47875Cysteine and glycine-rich protein 1214558.951%58/566/44−7.38−11.9NLDSTTVAVHGEEIYCK,
GLESTTLADKDGEIYCK
Cytoskeleton regulation
1323P47875Cysteine and glycine-rich protein 1214558.954%91/567/4013.7712.77TVYFAEEVQCEGNSFHK,
HEEAPGHRPTTNPNASK
Cytoskeleton regulation
416Q5XI50E3 ubiquitin-protein ligase MARCH7769327.6416%53/519/44−2.86−3.98MVSGNRGTSLNDSYHSR,
CTGSLQYVHQECMK
Protein degradation
709P62630Elongation factor 1-alpha 1504249.129%87/5611/35−2.01−1.1EHALLAYTLGVK,
STTTGHLIYK
Protein synthesis
1244O08719Ena/VASP-like protein421838.7420%59/516/432.271.63WVPIKPGQQGFSR,
VKPAGSVNDVGLDALDLDRMK
Cytoskeleton regulation
305Q99PF5Far upstream element-binding protein 2744666.3841%169/5620/43−2.09−2.25ERDQGGFGDR.
IGQQPQQPGAPPQQDYTK
Gene expression
886P97590Galectin-7153336.4329%52/516/43−1.41−1.58MPSSNVRSVEVGGDVQLHSVK,
MSATHHK
Apoptosis
1251Q9Z1B2Glutathione S-transferase Mu 5270676.3350%76/5615/54−4.85−9.44ITQSNAILR,
VDIMENQIMDFR
Redox regulation
1255P42930Heat shock protein beta-1229366.1256%147/5612/65−3.9−4.49KYTLPPGVDPTLVSSSLSPEGTLTVEA,
VPFSLLR
Chaperon
954Q6RUG5Islet cell autoantigen 1-like protein492985.2331%54/519/53−1.64−1.6MDSFEHLRPEDSQSVVSRMQK,
DASQELDPDTFK
Unknown
1240O35760Isopentenyl-diphosphate Delta-isomerase 1267215.5738%61/566/58−1.8−1.31MPEINASNLDEK,
AELGIPLEEVDLNEMNYLTR
Lipid synthesis
1237Q63279Keratin, type I cytoskeletal 19446095.2125%51/5110/734.023.7QGPGPFRDYSQYFK,
MSVEADINGLRR
Cytoskeleton
1219Q6QLM7Kinesin heavy chain isoform 5A1176425.5615%54/5112/43−2−2.3SLTEYMQTVELKK,
MAETNNECSIKVLCR
Transport
418P56536Kinesin heavy chain isoform 5C (Fragment)273765.8725%63/516/40−2.7−3.79FVSSPEEVMDVIDEGK,
NRHVAVTNMNEHSSR
Transport
349P48679Lamin-A745646.5448%221/5632/68−2.04−2.28LQDEMLRR,
LESSESR
Cytoskeleton
1263Q6AYP2Microfibrillar-associated protein 3-like458044.913%55/517/3012.22DEVYTIPNSLKR,
VTQFKTMEFAR
Sperm development
1355P13832Myosin regulatory light chain RLC-A199404.6762%102/5613/522.181.82DGFIDKEDLHDMLASMGK,
GNFNYIEFTR
Muscle contraction
1357Q64122Myosin regulatory light polypeptide 9197654.842%68/5110/482.121.5EAFNMIDQNR,
KGNFNYVEFTR
Muscle contraction
1276Q63716Peroxiredoxin-1 223238.2741%77/567/44−1.84−2.56ADEGISFR,
MSSGNAKIGHPAPSFK
Redox regulation
1213P97562Peroxisomal acyl-coenzyme A oxidase 2775487.6417%53/5110/412.191.96HGMHAFIVPIR,
LAWSLGWSEDGPER
lipid metabolism
1198P25113Phosphoglycerate mutase 1289286.6740%72/518/28−1.66−5.8YADLTEDQLPSCESLKDTIAR,
VLIAAHGNSLR
Glycolysis
1202P25113Phosphoglycerate mutase 1289286.6760%169/5122/591.411.56HGESAWNLENR,
FSGWYDADLSPAGHEEAK
Glycolysis
1405P62963Profilin-1151198.4675%94/5611/44−3.011.12EGVHGGLINK,
EGVHGGLINKK
Cytoskeleton regulation
1120P18420Proteasome subunit alpha type-1297846.1544%115/5612/28−1.37−1.53NQYDNDVTVWSPQGR
QECLDSR
Protein degradation
1242P40112Proteasome subunit beta type-3232356.1543%60/519/50−3.09−1.49LNLYELKEGR,
NCVAIAADRR
Protein degradation
1300P34067Proteasome subunit beta type-4293496.4534%70/5612/626.236.45FDCGVVIAADMLGSYGSLAR,
VNDSTMLGASGDYADFQYLK
Protein degradation
1321P28075Proteasome subunit beta type-5287386.5235%75/568/4815.0413.13GMGLSMGTMICGWDKR,
RGPGLYYVDSEGNR
Protein degradation
522P11598Protein disulfide-isomerase A3570445.8826%77/5611/381.311.69GFPTIYFSPANK,
IFRDGEEAGAYDGPR
Redox regulation
1301Q6IML7Rab and DnaJ domain-containing protein313298.7226%54/516/30−3.01−2.61EPLKSLR,
CIDESEGRLWAESR
Signal transduction
781P29315Ribonuclease inhibitor516534.6757%174/5618/64−1.56−1.42LSLQNCSLTEAGCGVLPDVLR,
LQLEYCNLTATSCEPLASVLR
Gene expression
952P62138Serine/threonine-protein phosphatase PP1-alpha catalytic subunit382295.9453%163/5616/49−1.44−1.52TFTDCFNCLPIAAIVDEK,
IYGFYDECK
Signal transduction
397P48721Stress-70 protein (mitochondrial)740975.9733%103/5621/79−2.12−4.1VCQGER,
DNMALQR
Chaperon
437O35814Stress-induced-phosphoprotein 1631586.420%88/5112/621.261.56AAALEFLNR,
TLLSDPTYR
Transport
1300P83941Transcription elongation factor B polypeptide 1126364.7445%56/565/626.236.45AMLSGPGQFAENETNEVNFR,
EIPSHVLSKVCMYFTYK
Gene expression
1208Q91Y78Ubiquitin carboxyl-terminal hydrolase isozyme L3262785.0163%124/5613/43−1.64−1.62HLENYDAIR,
VDLHFIALVHVDGHLYELDGR
Protein degradation