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Figure 1: FUZHENGHUAYU Tablet induced apoptosis in rat HSC-T6 cells. Cultured rat HSC-T6 cells were divided into control and drug groups. The control group was incubated with DMEM medium containing 10% control serum, and the drug group was cultured with DMEM medium supplemented 10% drug serum for up to 72 h. Apoptosis was assessed using an Annexin V/7-AAD Apoptosis kit and analyzed using a BD FACS Calibur flow cytometer. (a) HSC-T6 cells exposed to 10% FBS and stained with Annexin V(+)/7-AAD(+); (b) HSC-T6 exposed to 10% FBS + 2  L/mL actinomycin D and stained with Annexin V(+)/7-AAD(+); (c) HSC-T6 cells exposed to 10% FBS + 2  L/mL actinomycin D and stained with Annexin V(+)/7-AAD(−); (d) HSC-T6 cells exposed to 10% FBS + 2  L/mL actinomycin D and stained with Annexin V(−)/7-AAD(+); (e) HSC-T6 cells exposed to 10% control serum and stained with Annexin V(+)/7-AAD(+). (f) HSC-T6 cells exposed to with 10% drug serum and stained with Annexin V(+)/7-AAD(+); (g) the late apoptotic cells in the drug (10%  1%) and control groups (5%  1%) ( , * ); (h) the early apoptotic cells in the drug (43%  6%) and control groups (26%  4%) ( , * ); (i) total apoptotic cells in the drug (53%  6%) and control groups (31%  4%) ( , ** ).