Figure 2: Emodin and aloe-emodin reduce ERα protein levels. MCF-7 cells were treated with emodin or aloe-emodin in a dose-dependent manner for 24 h (a, c) and via a time course using 25 μM of the drug (b, d). ERα protein levels were detected by western blotting. β-Actin was used as an internal control. The quantification of ERα levels is presented as fold increase or decrease compared to control levels and was evaluated in three independent experiments. The results were presented as the mean SEM. * and ** correspond to , and , respectively, versus the control group.