376123.fig.006a
(a)
376123.fig.006b
(b)
376123.fig.006c
(c)
Figure 6: Effects of aloe-emodin and emodin on ERα subcellular distribution in comparison to EB. (a, b) MCF-7 cells were treated with aloe-emodin (25 μM) or estradiol benzoate ((EB) 10 nM) in the presence of MG132 (5 μM) for 24 h. The interaction between HSP90 and ERα was evaluated by ERα immunoprecipitation followed by western blotting of HSP90 with specific antibodies. Immunoprecipitation by IgG served as a negative control. The fractionation of cellular proteins was performed, and ERα protein was detected by western blotting. (c) MCF-7 cells were treated with emodin (25 μM) or EB (10 nM) in the presence of MG132 (5 μM) for 24 h. The fractionation of cellular proteins was performed, and ERα protein was detected by western blotting. PARP and α-tubulin served as markers for the nuclear and cytoplasmic fractions, respectively. The lysates were used in the western blotting experiments to indicate protein levels. β-Actin served as an internal control for western blotting.