Shikonin Suppresses Human T Lymphocyte Activation through Inhibition of IKKβ Activity and JNK Phosphorylation
Figure 7
Effect of shikonin on MAPK phosphorylation stimulated by PMA/ionomycin. The human T lymphocytes were pretreated with shikonin at 37°C for 60 min and then costimulated with PMA (20 ng/mL)/ionomycin (1 μM) (P/I) at 37°C for different time points. The whole-cell lysates were prepared, and proteins were analyzed by Western blotting using antibodies against ERK, JNK, and p38 and the phosphorylated forms of ERK, JNK, and p38 (a, b, and c). Data are representative of three independent experiments.
