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Evidence-Based Complementary and Alternative Medicine
Volume 2013 (2013), Article ID 385685, 9 pages
http://dx.doi.org/10.1155/2013/385685
Research Article

Chinese Medicine Formula “Jian-Pi-Zhi-Dong Decoction” Attenuates Tourette Syndrome via Downregulating the Expression of Dopamine Transporter in Mice

1Department of Pediatrics, Dongfang Hospital, Beijing University of Traditional Chinese Medicine, Beijing 100078, China
2Department of Nuclear Medicine, Chinese PLA General Hospital, Beijing 100853, China

Received 9 October 2012; Revised 9 December 2012; Accepted 3 January 2013

Academic Editor: Ki-Wan Oh

Copyright © 2013 Dao-han Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Jian-Pi-Zhi-Dong Decoction (JPZDD) is dedicated to the treatment for Tourette syndrome (TS) with the guidance of the theories of Traditional Chinese Medicine (TCM). This study aims to investigate the expression of dopamine transporter (DAT) in the striatum and stereotyped behavior of TS mice model by intervention of JPZDD. Mice were induced by 3,3′-iminodipropionitrile (IDPN, 350 mg kg−1 day−1, i.p.) for 7 days and divided into 4 groups ( , each): control and IDPN groups were gavaged with saline and the remaining 2 groups with Tiapride (Tia, 50 mg kg−1 day−1) and JPZDD (20 g kg−1 day−1), respectively. The results showed that the scores of stereotyped behavior in IDPN+JPZDD group were significantly reduced. A noticeably increased 11C-β-CFT binding at bilateral striatum was observed after administration of JPZDD versus that of IDPN or Tia. Immunohistochemistry and in situ hybridization studies manifested higher levels of DAT protein and mRNA in IDPN+JPZDD group. These findings not only demonstrated that JPZDD could effectively inhibit the abnormal behaviors of TS mice model, but also increase the level of DAT in striatum. Therefore, JPZDD could be one of potential treatments of patients with TS.