Chinese Medicine Formula “Jian-Pi-Zhi-Dong Decoction” Attenuates Tourette Syndrome via Downregulating the Expression of Dopamine Transporter in Mice

Figure 3

Immunostaining of DAT protein and in situ hybridization of DAT mRNA level in the striatum. Panel (A) displays the immunostaining of DAT protein (black arrow) in striatum in each group detected by immunohistochemistry. Magnification 100x. Panel (B) indicates the histogram of density of DAT protein. Data were represented as the mean ± SEM (𝑛=20 mice/group). Panel (C) displays the expressions of DAT mRNA (black arrow) in striatum from each group detected by in situ hybrization. Magnification 40x. Panel (D) indicates the histogram of DAT mRNA density. Data were indicated as the mean ± SEM (𝑛=20 mice/group). (a) Saline group, (b) IDPN group, (c) IDPN+Tia group, and (d) IDPN+JPZDD group. DAT protein and its mRNA were tagged with black arrow. ∗ indicates 𝑃<0.05 and ∗∗ indicates 𝑃<0.01 compared with IDPN group, respectively; ## indicates 𝑃<0.01 compared with saline group; ▲ indicates 𝑃<0.05 and ▲▲ indicates 𝑃<0.01 compared with IDPN+Tia group, respectively.