Research Article

Persea americana Mill. Seed: Fractionation, Characterization, and Effects on Human Keratinocytes and Fibroblasts

Table 1

Assay IDs (Life Technologies, Darmstadt) for gene expression analysis performed with Real-Time PCR. NHDF and NHEK were treated with 10  g/mL avocado seed HSCCC fractions. 18 s rRNA was used for endogenous control. KGFR and involucrin were only used for analysis of NHEK gene expression, while fibronectin 1 and collagen 1 gene expression was only measured in NHDF.

Investigated genes belong toAbbreviationNHDFNHEK

ProliferationSTAT6Hs00598618m1
InsRHs00169631m1
KGFR (FGFR2b)Hs00240796m1

Proliferation or differentiation (depending on stimulus)EGFR Hs01076068m1
FN1Hs00415006_m1

DifferentiationCol1A2Hs00164099_m1
InvHs00846307s1
SMAD3Hs00706299s1

Endogenous control18 sHs99999901s1