The Herbal Drug Melampyrum pratense L. (Koch): Isolation and Identification of Its Bioactive Compounds Targeting Mediators of Inflammation

Figure 2

HPLC-DAD (detection wavelength 254 nm) chromatogram of the detannified MeOH extract of Melampyrum pratense. The chromatographic separation was assessed on an Acclaim 120 C₁₈ reversed-phase column (150 mm × 2.1 mm i.d., 3 μm) equipped with an Acclaim 120 C₁₈ guard column (10 mm × 2.1 mm i.d., 5 μm) from Dionex (Germering, Germany) using the following mobile phase gradient of acetonitrile-water (A: H₂O + formic acid (pH = 2.5), B: MeCN): 2% B (5 min), in 5 min to 15% B, in 3 min to 21% B, in 27 min to 35% B, and in 10 min to 95% B at a flow rate of 0.4 mL/min and a temperature set to 35°C. The following compounds could be identified: (5) melampyroside, (6) luteolin, (7) apigenin, and (8) chrysoeriol.