Effect of Piper betle and Brucea javanica on the Differential Expression of Hyphal Wall Protein (HWP1) in Non-Candida albicans Candida (NCAC) Species

Table 1

Oligonucleotide sequences of HWP1 and ACT1.

Primersa	Sequences (5^′→3^′)^b	Temp (°C)	bpc
Forward (HWP1_For)	CCATGTGATGATTACCCACA	60.9	572
Reverse (HWP1_Rev)	GCTGGAACAGAAGATTCAGG	61.7
Forward (ACTI_For)	GGCTGGTAGAGACTTGACCAACCATTTG	67.6	304
Reverse (ACT1_Rev)	GGAGTTGAAAGTGGTTTGGTCAATAC	61.4

aPrimer templates are chosen as prescribed in Naglik et al. [20] and Tavanti et al. [21].
bMelting temperatures are analysed using Oligo Analyzer 1.2.
cRT-PCR product size (bp, base pair).