An Engineered Arginase FC Protein Inhibits Tumor Growth In Vitro and In Vivo
Figure 2
Enzymatic activity of rhArg-Fc. (a) and (b) native arginase or rhArg-Fc at the indicated concentration (nM) were incubated with 100 μM arginine (a) or asparagine (b) in PBS for 2 h at 37°C. The amino acid concentration was determined by HPLC, as described in Section 2, and normalized to the sample without drug treatment. For the time-course experiment of the effect of rhArg-Fc on arginine depletion, rhArg-Fc at different concentrations was added to RPMI medium supplemented with 100 μM of arginine. Each mixture with rhArg-Fc was divided into 5 groups and incubated at 37°C. The amino acids were extracted at the indicated time points, and the collected samples were quantified and normalized to the amount of the sample without rhArg-Fc treatment.
