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Evidence-Based Complementary and Alternative Medicine
Volume 2013 (2013), Article ID 439690, 12 pages
http://dx.doi.org/10.1155/2013/439690
Research Article

Metabolomic Study of Collagen-Induced Arthritis in Rats and the Interventional Effects of Huang-Lian-Jie-Du-Tang, a Traditional Chinese Medicine

1School of Pharmacy, Second Military Medical University, 325 Guo-He Road, Shanghai 200433, China
2College of Traditional Chinese Medicine, Jilin Agricultural University, Changchun 130118, China
3School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200030, China

Received 18 September 2012; Revised 28 January 2013; Accepted 28 January 2013

Academic Editor: Wei Jia

Copyright © 2013 Rongcai Yue et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Huang-Lian-Jie-Du-Tang (HLJDT) is a traditional Chinese medicine (TCM) with anti-inflammatory activity. The present study used a metabolomic approach based on LC-Q-TOF-MS to profile rheumatoid-arthritis- (RA-) related metabolic changes and to investigate the interventional mechanisms of HLJDT in collagen-induced arthritis rats. Forty male Wistar rats were randomly divided into five groups: (1) a model group, (2) a normal control group, (3) a dexamethasone group, (4) a HLJDT group, and (5) a group that received 13 components of HLJDT. Plasma samples were collected 8, 15, and 22 days after the rats were injected with bovine type II collagen. By combining variable importance in the projection values with partial least squares discriminant analysis, 18 potential biomarkers were identified in the plasma samples. The biomarkers were primarily involved in glycerophospholipid metabolism, fatty acid metabolism, tryptophan metabolism, linoleic acid metabolism, phenylalanine metabolism, purine metabolism, arachidonic acid metabolism, and bile acid biosynthesis. Using the potential biomarkers as a screening index, the results suggest that HLJDT can potentially reverse the process of RA by partially regulating fatty acid oxidation and arachidonic acid metabolism. This study demonstrates that a metabolomic strategy is useful for identifying potential RA biomarkers and investigating the underlying mechanisms of a TCM in RA treatment.