Hyperglycemia Induces Altered Expressions of Angiogenesis Associated Molecules in the Trophoblast
Figure 4
The effect of hyperglycemia on the expression of proinflammatory cytokines in 3A-Sub-E trophoblast cells. 3A-Sub-E cells were exposed to 30 mM D-glucose or to 5.6 mM of D-glucose (control) for 48 h. Cell lysates (250–370 μg) and the cultured conditioned media were collected for the antibody array analysis, respectively. (a) Representative dot image of the indicated proinflammatory cytokines. (b) Average intensity for each pair of cytokine spots was quantitatively measured by Scion Image software. Data are presented as mean SD (). Each cytokine is represented by duplicate spots as GRO (growth-regulated oncogene), IL-6 (interleukin-6), IL-8 (interleukin-8), and control (HRP-conjugated antibody as a positive control). *. **. n.s., not significant.
