Hyperglycemia Induces Altered Expressions of Angiogenesis Associated Molecules in the Trophoblast
Figure 5
The effect of hyperglycemia on the expression of C-C motif chemokines in 3A-Sub-E trophoblast cells. 3A-Sub-E cells were exposed to 30 mM D-glucose or to 5.6 mM of D-glucose (control) for 48 h. Cell lysates (250–370 μg) and the cultured conditioned media were collected for the antibody array analysis, respectively. (a) A representative dot image of the indicated C-C motif chemokines. (b) Average intensity for each pair of cytokine spots was quantitatively measured by Scion Image software. Each cytokine is represented by duplicate spots: MCP-1 (monocyte chemoattractant protein-1, CCL-2), RANTES (regulated upon activation, normal T-cell expression and secreted, CCL-5), and control (HRP-conjugated antibody as a positive control). Data are presented as mean SD (). **.
