Hyperglycemia Induces Altered Expressions of Angiogenesis Associated Molecules in the Trophoblast
Figure 7
The effect of hyperglycemia on the expression of perlecan binding growth factors in 3A-Sub-E trophoblast cells. 3A-Sub-E cells were exposed to 30 mM D-glucose or to 5.6 mM of D-glucose (control) for 48 h. Cell lysates (250–370 μg) and the cultured conditioned media were collected for the antibody array analysis, respectively. (a) A representative dot image of the indicated growth factors was shown. Compared to the control, only bFGF was expressed in the cell lysates. bFGF, basic fibroblast growth factor; VEGF, vascular endothelial growth factor; PDGF-bb, platelet derived growth factor-bb; VEGF-D, vascular endothelial growth factor D. (b) The mRNA expressions of bFGF in responses to heparinase (HepIII), chondroitinase ABC (Chabc), or both treatment in 3A-Sub-E cells under high (black bar) or normal glucose (white bar) culture condition. Compared to the control cells without any treatment at normal glucose condition, the mRNA level of bFGF was significantly increased in 3A-Sub-E cells cultured with HepIII, Chabc, or both treatments.
