PI3K/Akt Pathway Contributes to Neurovascular Unit Protection of Xiao-Xu-Ming Decoction against Focal Cerebral Ischemia and Reperfusion Injury in Rats
Figure 4
Representative images of neuron apoptosis of different groups in penumbra of ischemic cortex at 24 h after reperfusion. (a) Representative photomicrographs of immunofluorescence labeling with NeuN (red) and TUNEL (green) double staining. Nuclei were counterstained with DAPI (blue), and collocation of green and blue indicated TUNEL positive cell. At 24 h after reperfusion, a significant number of TUNEL-positive cells were observed in ischemic cortex of the I/R group and were strongly attenuated by XXMD treatment. (b) Quantification of apoptotic neurons. TUNEL and NeuN double stained cells (yellow) indicated the apoptotic neurons which were significantly induced by reperfusion and decreased by XXMD treatment. (c) Percentage of apoptotic neuron in NeuN positive cell. Percentage of apoptotic neuron was increased after stroke with reperfusion and was markedly reduced by XXMD treatment. However, neuron apoptosis was worsened by treatment with LY294002. LY, LY294002. Scale bar = 50 μm. Data are reported as the means ± SEM. ; * versus the I/R group.
