PI3K/Akt Pathway Contributes to Neurovascular Unit Protection of Xiao-Xu-Ming Decoction against Focal Cerebral Ischemia and Reperfusion Injury in Rats
Figure 7
Western blot analysis of phosphorylation levels of PDK1, PTEN at 24 h after reperfusion. (a) Representative protein bands for p-PDK1 (Ser241), total PDK1, were shown along with their corresponding relatively densities. Although the levels of p-PDK1 (Ser241) were significantly decreased at 24 h after reperfusion, XXMD treatment blocked such changes. However, the effects were partly blocked by LY294002. No changes in PDK1 were detected in rats of different groups. GAPDH was used to show equal protein loading of each lane. (b) Representative protein bands for p-PTEN (Ser380), total PTEN. Relative densities demonstrated that p-PTEN (Ser380) expression levels were decreased at 24 h after ischemia. Levels of p-PTEN (Ser380) in the XXMD-treated rats were significantly higher than those in the I/R group, suggesting that XXMD treatment upregulated protein levels of p-PTEN (Ser380) after stroke with reperfusion and LY294002 had no effect on its expression. No changes in PTEN were observed in rats of different groups. Data are reported as the means ± SEM. ; * versus the I/R group.
