PI3K/Akt Pathway Contributes to Neurovascular Unit Protection of Xiao-Xu-Ming Decoction against Focal Cerebral Ischemia and Reperfusion Injury in Rats
Figure 8
Western blot analysis of phosphorylation levels of Akt, GSK3β, and c-Raf at 24 h after reperfusion. (a) Representative protein bands for p-Akt (Ser473), total Akt. p-Akt (Ser473) expression levels significantly were decreased at 24 h after reperfusion, whereas XXMD treatment enhanced p-Akt (Ser473) levels and the effects could be partly reversed by PI3K inhibitor. No changes in Akt were detected in rats of different groups. GAPDH was used to show equal protein loading of each lane. (b) Representative protein bands for p-GSK3β (Ser9), total GSK3β. A decrease in p-GSK3β (Ser9) level was observed in the peripheral area of ischemia after reperfusion and the levels of p-GSK3β (Ser9) in the XXMD60 group were higher than those in the I/R group. However, inhibition of PI3K using LY294002 abolished the increase. No changes in GSK3β were observed in rats of different groups. (c) Representative protein bands for p-c-Raf, total c-Raf. Although p-c-Raf expression levels significantly were decreased at 24 h after reperfusion, XXMD preserved the levels of p-c-Raf. Notably, LY294002 did not significantly block the effect of XXMD on p-c-Raf. No changes in c-Raf were detected in rats of different groups. Data are reported as the means ± SEM. ; * versus the I/R group.
